• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of molecular structure and function of virulence gene of fish-pathogenic bacteria

Research Project

Project/Area Number 03660198
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General fisheries
Research InstitutionMiyazaki University

Principal Investigator

AOKI Takashi  宮崎大学, 農学部, 教授 (00051805)

Co-Investigator(Kenkyū-buntansha) 北尾 忠利  宮崎大学, 農学部, 教授 (60094075)
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1992: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1991: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsFish-pathogenic bacteria / Virulence gene / Hemolysin gene / Aeromonas hydrophila / Aeromonas salmonicida / Hemolysin / Analysis of molecular structure / Exotoxin / Aeromonas salmonicida
Research Abstract

Fish-pathogenic aeromonads produce several extracellular toxins of which hemolysin is thought to be one of the important virulence factors. In this study, the DNA structure and characterization of the hemolysin genes from Aeromonas hydrophila, A. salmonicida, and A. sobria have been examined.
Nine hemolysin genes have been cloned and their nucleotide sequences determined. Two(AHH1, AHH2) were from A. hydrophila strain ATCC7966, two(AHH3, AHH4) were from A. hydrophila strain 28SA, one(AHH5) was from A. hydrophila strain AH-1, one(ASH1) was from A. salmonicida strain ATCC 14174, two(ASH3, ASH4) were from A. salmonicida strain 17-2, and one(ASA1) was from A. sobria. The nucleotide sequences of the open reading frames and predicted molecular mass of the nines respectively were 1,731 base pair(bp) and 63,658 Daltons(Da), 978 be and 37,798 Da, 1,476 bp and 54,177 Da, 1476 bp and 54,159 Da, 1563 bp and 53,779 Da, 1716 bp and 64,780 Da, 1,467 bp and 54,188 Da, 1,734 bp and 63,400 Da, and 1,464 … More bp and 53,920 Da. The molecular mass predicted from the nucleotide sequences were similar to that of the molecular mass from maxicell analysis, in all except AHH2.
Hemolysin genes were classified into two groups depending on their nucleotide sequences. AHH1 and ASH4 belonged to one group which contained part of a homologous sequence of the hemolysin genes of Vibrio cholerae E1 Tor and V. vulnificus. This was designated group I and possessed the two highly conservative regions, and the location of cystein residue was conserved. regions may be important in the control of hemolytic activity. The other, group II is the previous reported aerolysin gene from A. hydrophila and A. trota, and AHH3, AHH4, AHH5, ASH3, and ASA1 were classified into this group. However AHH2 and ASA1 had no significant sequence homologies with those of the bacterial hemolytic proteins present in GENETYX-CD data bases.
AHH1 gene(group I) and the AHH5 and ASA1 genes(group II) were widely distributed in aeromonads.
Hemolysin was secreted by Escherichia coli containing each of the cloned genes except AHH2. Sensitivity to lysis by the cloned hemolysins was different for various species of erythrocytes as demonstrated by hemolysis on blood agar plates. Less

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] T.Aoki and I.Hirono: "Cloning of femolysin genes of aeromonads." NOAA Technical Reports NMFS. 111. 21-25 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] I.Hirono,T.Aoki,T.Asao,and S.Kozaki: "Nucleotide seguenves and Characterization of hemolysin genes from Aeromonas hydrophila and A.Sobria." Microb.Pathogen.13. (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] I.Hirono and T.Aoki: "Characterization of three different hemolysin genes from Aeromonas salmonicida." Microb.Pathogen.14. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Aoki and I.Hirono: "Comparison of amino acid seguences of cloned hemolysins from aeromonads and detection of cloned hemolysin gene in aeromonads." The Third Asian Fisheries Forum. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] I.Hirono and T.Aoki: "Diseases in Asian Aquaculture I (分担執筆)" Fish Health Section,Asian Fisheries Society,Manila,Philippines, 587 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Aoki: "Salmonid Diseases(分担執筆)" Hokkaido University Press,Sapporo,Japan, 326 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Aoki and I.Hirono: "Cloning and characterization of the haemolysin determinants from Aeromonas hydrophila" J.Fish Diseases. 14. 303-312 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] I.Hirono and T.Aoki: "Nucleotide sequence and expression of an extracellular hemolysin gene of Aeromonas hydrophila" Microbial Pathogensis. 11. 189-197 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] I.Hirono and T.Aoki: "Cloning and expression of a haemolysin gene from Aeromonas salmonicida ATCC 14174" Proceedings of the first Symposium on Diseases in Asian Aquaculture. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Aoki: "Hemolysin gene of Aeromonas Salmonicida" Oji International Symposium on Salmonid Diseases. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Aoki and I.Hirono: "Cloning of hemolysin genes of aeromonads" Proceedings of the 19th U.S.ーJapan Meetings on Aquaculture. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] I.Hirono,T.Aoki,T.Asao and S.Kozaki: "Nucleotide sequences and characterization of hemolysin genes from Aeromonas hydrophila and A.sobria" Infection and Immunity.

    • Related Report
      1991 Annual Research Report

URL: 

Published: 1991-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi