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Cell culture and application of fish cells with defferentiated functions

Research Project

Project/Area Number 03660202
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General fisheries
Research InstitutionNIHON UNIVERSITY

Principal Investigator

WATANABE Tasuku  Ph.D., Nihon University, Department of Agriculture and Veterinary Medicine, Associate Professor, 農獣医学部, 助教授 (30095517)

Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1992: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1991: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsLiver cells / Glycoprotein / Yamame / Hematopoietic cell / Stem cell / Semi-solid agar / Oncogene / 魚類 / 分化機能 / 細胞培養 / 培養肝細胞 / 血液幹細胞 / サイトカイン
Research Abstract

Yamame embryonal liver cell line, YEL-13, produces and releases large glycoprotein, which was stained with Giemsa and PAS. Electron microscopically, the cells had polymorphic unclei and well developed microfibril, and lost the morphological properties of liver parenchymal cells. Electron-dense graunles, surrounded by cell membrane, budded and were released from the surface of cells. Glycoprotein, isotlated from the conditioned medium of YEL-13, were purified partially with Parcoll zonal density gradient centrifugation and had the density of 1.04g/ml. Antiserum to this fraction stained the glycoprotein of YEL-13 and liver parenchymal cells of Yamame, strongly. These results suggest that YEL-13 became to produce these glycoprotein super-fluously with the immortalization in in vitro. We started the studies on the role of the glycoprotein in liver and on the analysis of sugar sequences using HPLC.
The hematopoietic stem cells of carp can multiply to from many colonies in semi-solid agar media when supplemented with carp serum. The colony forming cells varied from undifferentiated stem cells to differentiated granulocytes, neutrophils, baso-phils, and monocytes. When used the media supplemented with the serum of adjuvant-injected carp, more colonies were formed than with normal carp serum, As this hematopoietic stem cell culture system of carp is available for the investigation of fish colony stimulate factors (CSF), we started the purification studies of CFSs from carp sera. We also started the oncogene transfection study to carp hematopoietic cells for the immortilization of white blood cells.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Moritomo,T.,H.Noda,I.Yamaguchi,T.Watanabe: "Enhan cement of granulocytepoiesis by Frennd's complete adjuvaut in carp." 日本水産学会誌. 58. 1145-1149 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Moritomo,T.,A.Itou,I.Yamaguchi,T.Watanabe: "In vitro colony formation of carp granulocytes in a semi-solid ogarculture system" Fish Pathology.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Moritomo,T., T.Noda, I.Yamaguchi, and T.Watanabe: "Enhancement of granulo-cytepoiesis by Freund's complete adjuvant in carp" Nippon Suisan Gak-kaishi. 58-6. 1145-1149 (1192)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Moritomo,T., A,Itou, I.Yamaguchi, and T.Watanabe: "In vitro colony formation of carp granulocytes in a semi-solid agar culture system" Fish Pathol.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Moritomo,T.,H.Noda, I. Yamaguchi,T.Watanabe: "Enhancement of granulocytepoiesis by Freund′s complete adjurantin carp." 日本水産学会誌. 58. 1145-1149 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Moritomo,T.,A.Itou,I. Yamaguchi,T.Watanabe: "In vitro colony formation of carp granulocytes in a semi-solid agar culture system" Fish Pathology.

    • Related Report
      1992 Annual Research Report
  • [Publications] Tadaaki Moritomo: "In vitro colony fomation of carp granulocytes in a semi solid agar culture system" Developmental and Comparative Immunology.

    • Related Report
      1991 Annual Research Report
  • [Publications] Tadaaki Moritomo: "Enhancement of granulocytepoiesis by Freund's complete adjuvant in carp" 日本水産学会誌.

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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