| Project/Area Number |
03660290
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
畜産化学
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| Research Institution | TOHOKU UNIVERSITY |
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Principal Investigator |
ITOH Takatoshi Faculty of Agriculture, Professor, 農学部, 教授 (80005610)
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| Co-Investigator(Kenkyū-buntansha) |
SUGAWARA Hiroshi Faculty of Agriculture, Technician, 農学部, 教務職員 (80089797)
KITAZAWA Haruki Faculty of Agriculture, Research associate, 農学部, 助手 (10204885)
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1992: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1991: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Lactic acid bacteria / Interferon / Cell wall / Mitogenic activity / インターフェロン / マクロファージ / マクロファ-ジ / インタ-フェロン / アシドフィラス |
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| Research Abstract |
The cell wall components of L. delbrueckii subsp. bulgaricus and L.acidophilus were characterized after hydrolysis with lysozyme and N-acetylneuraminidase. The difference between two species were elucidated.
In order to evaluate the biological activity induced by L. acidophilus group lactic acid bacteria which can grow in intestinal tract, induction of interferon by L. acidophilus and L. gasseri was investigated in murine peritoneal macrophage cultures. Four strains in 9 L. acidophilus strains induced interferon in macrophage cultures. Such activation effect by L. gasseri was also formed on 5 strains in 13 strains. Induced interferons were characterized as alpha/beta by treatment with anti-interferon serum.
The biological activities of whole cell and hydrolyzed cell component of 9 L. acidophilus strains were evaluated by mitogenic activities using murine spleen cells. Higher activity was observed in only one whole cell, however, the cell wall components from 6 strains showed significantly higher activity. The cell wall component from one strain which showed the highest value was fractionated by ion-exchange chromatography, followed by measurement of the activity and chemical analysis. The activity was mainly found in the bound fractions and it was confirmed to be specific on B-cell of murine spleen cells. Carbohydrate moiety of the active fractions was composed of glucose, N-acetylglucosamine, N-acetylmuramic acid and glycerol. Higher active fraction tends to contain higher N-acetylglucosamine in the ratio against glucose.
The results indicate that some strains of L. acidophilus group can act as an immunostimulators in the intestinal tract when dairy products containing such lactic acid bacteria was ingested.
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