DNA Diagnosis of Porcine Stress Syndrome

• Project/Area Number: 03660295
• Research Category: Grant-in-Aid for General Scientific Research (C)
• Allocation Type: Single-year Grants
• Research Field: 畜産化学
• Research Institution: Kyushu University
• Principal Investigator: ITO Tatsumi Kyusyu University, Animal Science, Profesor, 農学部, 教授 (50038246)
• Project Period (FY): 1991 – 1993
• Project Status: Completed (Fiscal Year 1993)
• Budget Amount: ¥1,900,000 (Direct Cost: ¥1,900,000); Fiscal Year 1993: ¥100,000 (Direct Cost: ¥100,000); Fiscal Year 1992: ¥200,000 (Direct Cost: ¥200,000); Fiscal Year 1991: ¥1,600,000 (Direct Cost: ¥1,600,000)
• Keywords: PSS / DNA Diagnosis / Malignant Hyperthermia / Ryanodine Receptor / PCR / ライアノジンレセプター / ストレス感受性琢 / ストレス抵抗性琢

Research Abstract

In order to elucidate the molecular genetics of porcine stress syndrome or porcine malignant hyperthermia developed by the malfunction of calcium release channel, ryanodine receptor, of porcine sarcoplasmic reticulum, sequences of foot ragion and channel portions of ryanodine receptor genomic DNA were propagated b PCR method and were analyzed with restriction enzyme's digestion and with Southern-hybridization method. It was found that there is at least one mutation site in the foot region of ryanodine receptor gene. In addition, a longer DNA fragment was formed during PCR-propagation. This indicated that there is at least one intron in the foot region of ryanodine receptor genomic DNA.Also, a similar mutation was found in PCR-products of genomic DNA of halothane-negative pigs, suggesting that molecular analysis of genomic DNA is more sentitive to detect mutations in stress-susceptable pigs. Present results suggest that a gateway for developing a mothod of DNA dagnosis of porcine stress syndrome will be opne shortly.