Genetic analysis of swine pneumonic mycoplasmas by PCR.

• Project/Area Number: 03660305
• Research Category: Grant-in-Aid for General Scientific Research (C)
• Allocation Type: Single-year Grants
• Research Field: 基礎獣医学
• Research Institution: University of Tokyo
• Principal Investigator: HARASAWA Ryo University of Tokyo, Faculty of Medicine, Associate Professor, 医学部(医), 助教授 (70159101)
• Project Period (FY): 1991 – 1992
• Project Status: Completed (Fiscal Year 1992)
• Budget Amount: ¥1,900,000 (Direct Cost: ¥1,900,000); Fiscal Year 1992: ¥800,000 (Direct Cost: ¥800,000); Fiscal Year 1991: ¥1,100,000 (Direct Cost: ¥1,100,000)
• Keywords: Mycoplasma / PCR / Swine enzootic pneumonia / ポリメラ-ゼ連鎖反応

Research Abstract

This study is focused on genetic analysis of Mycoplasma hyopneumoniae which is considered as an etiologic agent of the enzootic pneumonia in swine. The 16S-23S rRNA intergenic spacer regions of M. hyopneumoniae strain VPPll was amplified by PCR and sequenced by dideoxy chain termination method. The 16S-23S spacer region revealed following features: (1) lack of tRNA genes, (2) existence of "boxA"-like sequence, (3) promotor-like structure, (4) nested-palindromic sequences, (5) antitermonator structure.

Research Products

• [Publications] HARASAWA,R.,et al.: ""boxA"-like sequence between the 16s/23s spacer in rRNA operon of mycoplasmas." FEBS Letters. 297. 209-211 (1992)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] UEMORI,T.,et al.他3名: "Amphitication of the 16s-23s spacer region in rRNA operon of mycoplasmas by the polymerase chain reaction." Systematic and Applied Microbiology. 15. 181-186 (1992)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] HARASAWA,R.et al 他4名: "Nucleotide sequence of the arginine deiminase gene of Mycoplasma hominis." Microbiology and Immunology. 36. 661-665 (1992)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] IMADA,Y.et al 他3名: "Sodium dodecyl sulfate-polyacrylamide gel electrophoreses and western bltting analyses of ureaplasmas isolated from dogs." Journal of Veterinary Sciences. 54. 325-328 (1992)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Harasawa, R. et al.: ""boxA"-like sequence between the 16S/23S spacer in rRNA operon of mycoplasmas." FEBS Letters. 297. 209-211 (1992)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Uemori, T., et al.: "Amplification of the 16S-23S spacer region in rRNA operon of mycoplasmas by the polymerase chain reaction." Systematic and Applied Microbiology. 15. 181-186 (1992)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Harasawa, R. et al.: "Nucleotide sequence of the arginine deiminase gene of Mycoplasma homins." Microbiology and Immunology. 36. 661-665 (1992)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Imada, Y. et al.: "Sodium dodecyl sulfate-poly-acrylamide gel electrophoresis and western blotting analyses of ureaplasmas isolated from dogs." Journal of Veterinary Sciences. 54. 325-328 (1992)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Harasawa,R.,et al.他4名: "“boxA"-like sequence between the 16S/23S spacer in γRNA operon of mycoplasmas." FEBS Letters. 297. 209-211 (1992)
• [Publications] Uemori,T.,et al.他3名: "Amplification of the 16S-23S spacer region in γRNA operon of mycoplasmas by the polymerase chain reaction." Systematic and Applied Microbiology. 15. 181-186 (1992)
• [Publications] Harasawa,R.,et al.他4名: "Nucleotide sequence of the arginine deiminase gene of Mycoplasma hominis." Microbiology and Immunology. 36. 661-665 (1992)
• [Publications] Imada.Y.,et al.他3名: "Sodium dodecyl sulfate-polyacrylamide gel electrophorisis and western blotting analyses of ureaplasmas isolated from dogs." Journal of Veterinary Sciences. 54. 325-328 (1992)
• [Publications] 原澤亮 他3名: "マイコプラズマ研究" 日本マイコプラズマ学会, 112 (1992)
• [Publications] Harasawa,R.,Koshimizu,K.,Takeda,O.,Uemori,T.,Asoda,K.,and Kato,I.: "Detection of Mycoplasma hyopneumoniae DNA by the polymerase chain reaction." Molecular and Cellular Probes. 5. 103-109 (1991)
• [Publications] Harasawa,R.,Dybvig,K.,Watson,H.L.and Cassell,G.H.: "Two geromic clusters amorg 14 serovars of Ureaplasma urealyticum." Systematic and Applied Microbiology. 14. 393-396 (1991)
• [Publications] Yoshida,T.,Harasawa,R.,and Goto,T.: "Translation of doubleーstranded RNA segments 1 and 2 of chuzan virus in rabbit reticulocyte lysate." Journal of Veterinary Medicine,series B.38. 630-634 (1991)
• [Publications] 原澤 亮,志賀 淳治,阿部 賢治: "PCR法の基礎と応用。" ビフィズス. 4. 141-144 (1991)
• [Publications] 原澤 亮 水澤 博: "PCR法によるマイコプラズマ汚染の検出" 実験医学. 9. 95-99 (1991)
• [Publications] Harasawa,R.,Uemori,T.,Asada,K.,and Kato,I.: "'BoxA'like seguence between the 16S/23S spacer in rRNA operon of mycoplasmas." FEBS Letters. 297. 1-3 (1992)