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Basic Research on Protective Immunity against Experimental Hemoprotozoan Infections

Research Project

Project/Area Number 03660318
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Applied veterinary science
Research InstitutionThe Research Center for Protozoan Molecular Immunology, Obihiro University

Principal Investigator

IGARASHI Ikuo  Obihiro Univ., Res.Ctr.Protozoan Mol.Immunol., Assoc.Prof., 原虫病分子免疫研, 助教授 (80159582)

Co-Investigator(Kenkyū-buntansha) OMATA Yoshitaka  Obihiro Univ., Vet.Physiol., Assoc.Prof., 畜産学部, 助教授 (10132987)
SAITO Atsushi  Obihiro Univ., Vet.Physiol., Prof., 畜産学部, 教授 (10002263)
SUZUKI Naoyoshi  Obihiro Univ., Res Ctr.Protozool.Mol.Immunol., Prof., 原虫病分子免疫研, 教授 (10003071)
Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1993: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1992: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1991: ¥900,000 (Direct Cost: ¥900,000)
KeywordsMurine Babesia / Protective immunity / CD4^+ T cells / Gamma interferon / Cross-reactive antigen / Renal pathology / Immunopathology / Nonspecific immunity / バベシア原虫 / 感染防御 / CD_4陽性細胞 / ガンア-インターフェロン / 赤血球内寄生原虫 / 感染防抑 / CD4^+細胞 / 感染防御免疫
Research Abstract

Role of cell-mediated immunity in Babesia infection was investigated using mouse models in the present study. In lethal Babesia rodhaini infection, drug-cured mice showed 75% protection against challenge infection with B.rodhaini and a humoral immunity appears to be major role in the effector phase of immunity. Mice which were cured first infection with anti-Babesia durg and survived with challenge infection, showed 100% protection against B.radhaini infection, Lyt1^+ and Lyt2^+ T cells may contribute in controlling secondary infection.
In B.microti infection, while mice sufferd high parasitemia, but recovered naturally from their primary infection, nu/nu mice and SCID had a higher peak parasitemia and failed to clear the infection. These results demonstrated that T lymphocytes are necessary for the resolution of B.microti infection. Mice depleted of CD4^+ T cells with monoclonal antibody (mAb) had high parasitemia and failed to control the infection, while depletion of CD8^+ cells had … More no effect on the course of infection. Culture supernatants of spleen cells from control and CD8^+ cells depleted, but not from CD4^+ depleted mice, showed high concentration of IFN-gamma. Treatment of infected mice with anti-IFN-gamma mAb showed higher parasitemia than that of the control group. These results suggest that IFN-gamma produce by CD4^+ T cells, at least in part, is responsible for the control of acute infection with B.microti in mice.
Mab, recognizing 70,30 kDa antigens of B.microti and challenged with B.rodhaini, was produced from mice infected with B.microti and challenged with B.rodhaini. Administration of the mAb gave the delay of onset of parasitemias in mice. Mice which were exposed to oocysts of Isospora felis, showed absolute resistance against B.microti infection on 28th day post-exposure of oocysts. CD4^+ T cells induced by I.felis infection provides mice protection against B.microti.
Pathological changes were examined in the kidneys and liver of mice of two babesioses. Severe damages to kidneys and liver were observed in B.rodhaini infection rather than in B.microti infection. Electron-dense deposits were demonstrated in the mesangial matrix and along the glomerular basement membrane and these deposits were suggested as immune complexes by immunohistochemistry. Less

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Shimada,T.,Igarashi,I.,Maki,Y.,Clavena,F.G.,Saito,A.and Suzuki,N.: "Cellular subsets involved in protective immunity to Babesia rodhaini infection in BLAB/c mice." Journal of Protozoology Research. 1. 35-44 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Takahasi,M.,Omata,Y.,Oikawa,H.,Claveria,F.G.,Igarashi,I.,et al.: "Protective immune response of lsospora felis infected mice against Babesia microti infection." Joumal of Veterinary Medical Science. 55. 587-590 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Igarashi,I.,Hosomi,T.,Kaidoh,T.,Omata,Y.,Saito,A.et al: "Comparison of damage to kidneys and liver caused by lethal Babesia rodhaini infection and non-lethal Babesia microtiinfectin in mice." Journal of Protozoology Research. 3. 144-155 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Inoue,N.,Omata,Y.,Igarashi,I.,Saito,A.,Claveria,F.G.and Suzuki,N: "Babesia rodhaini and Babesia microti.Cross-Immunity and cross-Antigens." Journal of Protozoology Research. 4. 98-104 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Igarashi,I.,Waki,S.,Ito,M.,Omata,Y.,Saito,A.and Suzuki,N.: "Role of CD4^+T cells in the control of primary infecttion with Babesia microti in mice." Journal of Protozoology Research. 4(in press).(1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Shimada, T., Igarashi, I., Maki, Y., Claveria, F.G., Saito, A.and Suzuki, N.: "Cellular subsets involved in protective immunity to Babesia rodhaini infection in BLAB/c mice." J.Protozool.Res.1. 35-44 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Takahashi, M., Omata, Y., Oikawa, H., Claveria, F.G., Igarashi, I., Saito, A.and Suzuki, N.: "Protective immune response of Isospora felis infected mice against Babesia microti infection." J.Vet.Med Sci.55. 587-590 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Igarashi, I., Hosomi, T., Kaidoh, T., Omata, Y., Saito, A.Suzuki, N.and Aikawa, M.: "Comparison of damage to kidneys and liver caused by lethal Babesia rodhaini infection and non-lethal Babesia microti infection in mice." J.Protozool.Res.3. 144-155 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Inoue, N., Omata, Y., Igarashi, I., Saito, A., Claveria, F.G.and Suzuki, N.: "Babesia rodhaini and Babesia microti : Cross-Immunity and Cross-Antigens." J.Protozool.Res.4. 98-104 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Igarashi, I., Waki, S., Ito, M., Omata, Y., Saito, A.and Suzuki, N.: "Role of CD4^+ T cells in the control of primary infection with Babesia microti in mice." J.Protozool.Res.(in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Takahashi: "Protecrive immune responses of Isospora felis infected mice against Babesia microti infection." Journal of Veterinary Medical Science. 55. 587-590 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] I.Igarashi: "Comparison of damage to kidneys and liver caused by lethal Babesia rodhaini infection and non-lethal Babesia microti infection in mice" Journal of Protozoology Research. 3. 144-155 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] K.Miyahara: "Antitumor activity of Toxoplasma lysate antigen against methycholanthrene-induced tumor bearing rats." Journal of Veterinary Medical Science. 54. 221-228 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Y.Fujii: "Effects of Obiopetide and Obioactin on the toxoplasmscidal activity,glucose consumption and ruffle formation in mouse macrophages." Journal of Vaterinary Medical Science. 54. 351-353 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] I.Igarashi: "Enzyme-linked immunosorbent assay(ELISA)using urease-conjugated antibodies for Toxplasma antibody detection." Journal of Veterianry Midical Science. 54. 585-587 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Terumasa SHIMADA: "Celluler Subsets Involved in Protective Immunity to Babesia rodhaini Infection in BALB/c Mice" Journal of Protozoology Research. 1. 35-44 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] A.Saito: "Effects of reactive oxygen intermediate scavengers on the antitoxoplasmic activity of activated macrophages" Parasitology Research. 78. 28-31 (1992)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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