| Project/Area Number |
03660322
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied veterinary science
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| Research Institution | YAMAGUCHI UNIVERSITY |
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Principal Investigator |
TAURA Yasuho FACULTY OF AGRICULTURE,YAMAGUCHI UNIVERSITY DEPARTMENT OF VETERINARY MEDICINE ASSOCIATE PROFESSOR, 農学部, 助教授 (80163153)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAMA Sanenori FACULTY OF AGRICULTURE,YAMAGUCHI UNIVERSITY DEPARTMENT OF VETERINARY MEDICINE PR, 農学部, 教授 (90081596)
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1992: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | FETAL PANCREAS / PANCEAS TRANSPLANTATION / XENOGRAFT / ULTRAVIOLET IRRADIATION / DIABETES / DOG / IMMUNOALTERATION / 異種移植 / ドナ-処理 |
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| Research Abstract |
THE PURPOSE OF THIS STUDY IS TO EXAMINE WHETHER FETAL PANCREATIC XENO-GRAFTS CAN EXERT THEIR EFFECT ON THE DIABETIC RECIPIENTS TO USING BY DIRECT ULTRAVIOLET (UV)-IRRADIATION OF THE DONORS IN VIVO AND TO EXAMINE BY THE MIXED ISLETS AND LYMPHOCYTE CULTURE(MILC)IN VITRO. [MATERIALS AND METHODS] ELEVEN TOTAL PANCREATECTOMIED MONGREL DOGS WERE USED TO THE RECIPIENTS OF UV-TREATED FETAL PANCREAS TRANSPLANTATION OR TO THE CONTROL GROUP.THE FETAL PANCREAS TISSUES WERE ENGRAFTED INTO OMENTUM POUCH OR SPLEEN AFTER DIRECT UV-IRRADIATION(UV LIGHT: UVM-57,302NM, UVP INC.,IRRADIATION TIME: 10MIN). [RESULTS] DIRECT UV-IRRADIATION OF FETAL PANCREAS PREVENTED THEM FROM INITIATING PROLIFERATIVE RESPONSES IN XENO-GENEIC MILC.THE RESPONSE REDUCED TO 73.4*8.8%(MEAN*SE)WITH GOOD VIA-BILITY COMPARED WITH NO UV-IRRADIATION.AT THE SURVIVAL DAYS FROM TOTAL PANCREATECTOMY WERE 84.2*14.1 DAYS IN XENOGRAFT.THE OTHER SIDE, THEY WERE SURVIVED AT 24.6*6.5 DAYS AND MORE THAN 252.2(] SY.-+.[)66.1 DAYS,IN NON-TRANSPLANTATION AND ALLOGRAFT RESPECTIVELY.DAILY INSULIN REQUIREMENTS, URINE GLUCOSE LEVELS WERE TRENDED TO DECREASE AFTER ZENO-TRANSPLANTATION. [CONCLUSION] THE ALTERATIONS IN IMMUNE RESPONSIVENESS RESULTING FROM THE USE OF UV-TREATED XENOGENEIC FETAL PANCREAS MAY ALLOW LONG-TERM GRAFT SURVIVAL WITHOUT REQUIRING LARGE DOSES OF IMMUNOSUPPRESSIVE DRUGS.
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