| Project/Area Number |
03670004
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
General anatomy (including Histology/Embryology)
|
|---|
| Research Institution | CHIBA UNIVERSITY |
|---|
Principal Investigator |
TOYOTA Fumie CHIBA UNIVERSITY SCHOOL OF MEDICINE, DEPARTMENT OF ANATOMY, ASSOCIATE PROFESSOR, 医学部, 助教授 (60009751)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MAEKAWA Mamiko CHIBA UNIVERSITY SCHOOL OF MEDICINE, DEPARTMENT OF ANATOMY, RESEARCH ASSISTANT, 医学部, 助手 (20181571)
NAGANO Toshio CHIBA UNIVERSITY SCHOOL OF MEDICINE, DEPARTMENT OF ANATOMY, PROFESSOR, 医学部, 教授 (60009082)
|
|---|
| Project Period (FY) |
1991 – 1993
|
| Project Status |
Completed (Fiscal Year 1993)
|
| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1993: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1992: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1991: ¥900,000 (Direct Cost: ¥900,000)
|
|---|
| Keywords | capacitation / boar spermatozoa / freeze-fracture / mouse spermatozoa / zona pellucida recognition and binding protein / immuno-cytochemistry / confocal laser scanning microscope / labeled surface replica / SP56 / 抗SP56モノクローナル抗体 / 金コロイド標識レプリカ法 / 膜内粒子 / 膜ステロール / フィリピン / 精子 / 膜ステロ-ル / ブタ / ヒト |
|---|
| Research Abstract |
(1) Changes of the plasma membtane of boar spermatozoa durin capacitation : The distribution change of intramembranous particles (IMP) in the periacrosomal plasma membrane (PAPM) of the boar spermatozoa was studied by freeze-fracture, During capacitation, IMP and filipin-sterol-complex free areas expanded. Statistical analysis of the IMP density suggested that small IMPs largely disappeared, while the majority of the large IMPs persisted in the PAPM.These results suggest that the small particles and the filipin reactive sterols act as membrane stavilizers to prevent a precocious acrosome reaction, and have to ve temoved furing capacitation.
(2) Distribution of egg recognition and binding protein, sp56, on the mouse spermatozoa : SP56 has stecific affinity for ZP3, one of the three glycoproteins constituting the zona pillucida( Bleil and Wassarman, 1990). In collaboration with the Dr. Bleil's Lab, we demonstrated by immuno-cytochemical study using a confocal microscope that sp56 molecules are localized on the crescent dorsal edge of the ouse spermatozoon, where is well corresponded with the reported ZP3 binding site. In EM level, we debeloped a method it has been demonstrated that the sp56 is absent in the tip of the hooked-shaped mouse sperm-head, and present in the outer surface of the plasma memvrace covering the dorsal edge.
|
