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Morphological studies of spermatozoa during capacitation and in relation to attachment mechmism with zona pellucida.

Research Project

Project/Area Number 03670004
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General anatomy (including Histology/Embryology)
Research InstitutionCHIBA UNIVERSITY

Principal Investigator

TOYOTA Fumie  CHIBA UNIVERSITY SCHOOL OF MEDICINE, DEPARTMENT OF ANATOMY, ASSOCIATE PROFESSOR, 医学部, 助教授 (60009751)

Co-Investigator(Kenkyū-buntansha) MAEKAWA Mamiko  CHIBA UNIVERSITY SCHOOL OF MEDICINE, DEPARTMENT OF ANATOMY, RESEARCH ASSISTANT, 医学部, 助手 (20181571)
NAGANO Toshio  CHIBA UNIVERSITY SCHOOL OF MEDICINE, DEPARTMENT OF ANATOMY, PROFESSOR, 医学部, 教授 (60009082)
Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1993: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1992: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1991: ¥900,000 (Direct Cost: ¥900,000)
Keywordscapacitation / boar spermatozoa / freeze-fracture / mouse spermatozoa / zona pellucida recognition and binding protein / immuno-cytochemistry / confocal laser scanning microscope / labeled surface replica / SP56 / 抗SP56モノクローナル抗体 / 金コロイド標識レプリカ法 / 膜内粒子 / 膜ステロール / フィリピン / 精子 / 膜ステロ-ル / ブタ / ヒト
Research Abstract

(1) Changes of the plasma membtane of boar spermatozoa durin capacitation : The distribution change of intramembranous particles (IMP) in the periacrosomal plasma membrane (PAPM) of the boar spermatozoa was studied by freeze-fracture, During capacitation, IMP and filipin-sterol-complex free areas expanded. Statistical analysis of the IMP density suggested that small IMPs largely disappeared, while the majority of the large IMPs persisted in the PAPM.These results suggest that the small particles and the filipin reactive sterols act as membrane stavilizers to prevent a precocious acrosome reaction, and have to ve temoved furing capacitation.
(2) Distribution of egg recognition and binding protein, sp56, on the mouse spermatozoa : SP56 has stecific affinity for ZP3, one of the three glycoproteins constituting the zona pillucida( Bleil and Wassarman, 1990). In collaboration with the Dr. Bleil's Lab, we demonstrated by immuno-cytochemical study using a confocal microscope that sp56 molecules are localized on the crescent dorsal edge of the ouse spermatozoon, where is well corresponded with the reported ZP3 binding site. In EM level, we debeloped a method it has been demonstrated that the sp56 is absent in the tip of the hooked-shaped mouse sperm-head, and present in the outer surface of the plasma memvrace covering the dorsal edge.

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Crichton E G,Suzuki F,Krutzsch P H,Hammerstedt R H: "Unique features of the cauda epididumal epithelium of hibernating bats may promote sperm longevity." Anat.Rec.237. 475-481 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Cheng A,Le T,Palacios M,BookbinderLH,WassarmanPM,Suzuki F,Bleil JD: "Sperm-egg recognition in the mouse:characterization of SP56,a sperm protein having specific affinity for ZP3." J.Cell Biol.(Submitted).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] F.Suzuki: "Ultrastructural changes of sperm plasma membrane during epididymal transport & in vitro capacitation:A freeze-fracture study;in:Comparative Spermatology 20 Years After" Ed.B.Baccetti,Raven Press,New York, 1112(5) (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Suzuki(Toyota) F: "Ultrastructural changes of sperm plasma membane during epididymal transport & in vitro capacitation : A freeze-fracture study" in Comparative Spermatology 20 years after B.Bccetti ed. Raven Press, NY. 693-697 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Cheng A, Suzuki(Toyota) F, Beleil JD et al: "Sperm0egg recognition in the mouse : characterization of SP56" a sperm protein having specific afinity for ZP3. J.Cell Biol. (submitted). 693-697

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Seki N, Toyama Y, Nagano,T: "Changes in the distribution of filipin-sterol complexes in the boar sperm head plasma membrane during epididymal maturation and in the uterus." Anat Rec. 232. 221-230 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Haneji T, Nagano T, Koide S S: "Identification of pyruvate carboxylase in 3T3-L1 cells by transblot and its correlation with differentiation into adipocytes." Biochem Int. 25. 995-1001 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Nagano T, Maekawa M: "Destribution of actin-filament bundles in myoid cells and Sertoli cells of the rat testis." Chonnam J Med Sci. 5. 66-69 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Suzuki F (Suzuki=Toyota): "Ultrastructural changes of sperm plasma membrane during epididymal transport and in vitro capacita tion.In; Comparative spermatology 20 years after" Serono Symposia Publications from Raven Press Vol 75 (B Baccetti ed), 1112(5) (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Nagano T, Maekawa M, Nakamura Y, Sakiyama S: "Stage-specific localization of cytoskeletal actinm-RNA in spermatogenesis of some rodents as observed by in-situ hybridization. In; same as above" Serono Symposia Publications from Raven Press Vol 75 (B Baccetti ed), 1112(4) (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] F.Suzuki&T.Nagano: "Three‐dimensional model of tight junction fibrils based on freeze‐fracture images." Cell Tiss.Res.264. 381-384 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] M.Maekawa,T.Nagano,K.Kamimura,T.Murakami,H.Ishikawa&M.Dezawa: "Distribution of actin‐filament bundles in myoid cells,Sertoli cells,and tunica albuginea of rat and mouse testes." Cell Tiss.Res. 266. 295-300 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] M.Maekawa&Y.Mishimune: "In vitro proliferation of germ cells and supporting cells in the neonatal mouse testis." Cell Tiss.Res.265. 551-554 (1991)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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