| Project/Area Number |
03670012
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Kochi Medical School |
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Principal Investigator |
OKADA Teruhiko Kochi Medical School, associate professor, 医学部, 助教授 (00025628)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Toshihiro Kochi Medical School, 医学部, 助手 (40153621)
SEGUCHI Harumichi Kochi Medical School, 医学部, 教授 (90030866)
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1992: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1991: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Stria vascularis / Cationized ferritin / Histochemistry / ACPase / TMPase / Na-K-ATPase / Ca-ATPase / Mg-ATPase / Mg-ATPase / CaーATPase / MgーATPase / NaーKーATPase |
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| Research Abstract |
The localization of ion-transporting enzyme activity and the endocytic process of cationized ferritin (CF) were electron microscopically investigated in the guinea pig stria vascularis.
Na-K-ATPase activity was localized in basolateral infolding of marginal cells of the sria vascularis. Ca-ATPase activity was detected in the limiting membrane of the large endosomal vacuoles and the luminal plasma membrane of the marginal cells. Mg-ATPase activity was found in the lateral plasma membrane of the marginal cells.
CF was first observed scattered on the luminal surface of the marginal cells. Then it was internalized in the form of coated vesicles and transported to the large vacuolar endosomes. At 5 min after CF injection, CF particles were mainly localized in the coated vesicles, and at 15 min, they appeared in large vacuoles. At 60 min, multivesicular bodies provided with several internal vesicles were havily labeled with CF.At 2 hours, the content of the multivesicular bodies condensed and internal vesicles, a granular substance of moderate to high density and membrane remants become clearly visible. These multivesicular bodies were havily labeled with clustered CF particles.
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