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Enzyme-histochemical method for identification of lymphatic vessels and its application

Research Project

Project/Area Number 03670014
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General anatomy (including Histology/Embryology)
Research InstitutionOita Medical University

Principal Investigator

KATO Seizi  Dept. of Anatomy, associate professor, 医学部(解剖学), 助教授 (60034956)

Co-Investigator(Kenkyū-buntansha) CHIKAMA H.  Dept. of Surgery, assistant professor, 医学部(外科学), 助手 (40244165)
YASUNAGA A.  Dept. of Surgery, assistant professor, 医学部(外科学), 助手 (10210270)
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1992: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1991: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsLymphatics / 5'-nucleotidase / enzyme histochemistry / Blood vessel / Alkaline phosphatase / microcirculation / SEM / TEM / 5'ーヌクレオチダ-ゼ / アルカル性フォスファタ-ゼ
Research Abstract

Enzyme-histochemical method to distinguish lymphatic capillaries from blood capillaries was established on the sections and whole mount preparations of animal and human tissues on the basis of the characteristic of 5'-nucleotidase(5'-Nase) and alkaline phosphatase (ALPase). The 5'-Nase-ALPase double staining method could demonstrate satisfactory isolated visualization of 5'-Nase activity in lymphatic capillaries and of ALPase activity in blood capillaries under light microscopy. The intensity and localization of 5'-Nase activity on the walls of lymphatic capillaries were also determined by the lead-based or cerium-based method under transmission electron microscopy. The intense reaction products of 5'-Nase activity were predominantly deposited on the outer surface of the plasma membrane of the lymphatic endothelial cells. Furthermore, the applicability of backscattered electron imaging of 5'-Nase reaction to SEM histochemistry of lymphatic capillaries stained with heavy metal was demonstrated on the tissue surface.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] YASUNAGA,A.,KATO,S.UCHIDA,Y.and MIYAUCHI,R.: "Enzyme-histochemical study on the fine distribution of the intramural lymphatics at the ileocecal junction of the monkey intestine." Okajimas Folia Anat.Jpn.68. 259-270 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] KATO,S.,YASUNAGA,A.and UCHIDA,Y.: "Enzyme-Histochemical method for identification of of lymphatic capillaries." Lymphology. 24. 125-129 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 安永 昭,内田 雄三 加藤 征治: "ヒト大腸粘膜固有層の微小循環系の酵素組織化学的検索" リンパ学. 14. 13-17 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kato,S.,Yasunaga,A.: "Enzyme-and immunohistochemical visualization of lymphatic capillaries in the mammalian thyroid gland:Light and electron microscopic study." Progress in Lymphology-XIII. 39-40 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kato,S.,Yasunaga,A.Uchida,Y.: "Ultrastructural localization of 5'-nucleotidase in the lymphatic endothelium." Progress in Lymphology-XIII. 41-42 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kato,S.,Yamada,M.,Gotoh,M.: "Lymphatic imaging of the enzyme-histochemical reaction by light and backscattered image scanning electron microscopy." Progress in Lymphology-XIII. 65-66 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 加藤 征治(分担) 監修 宮澤 七郎/安達 公一: "医学・生物学の走査電子顕微鏡(分担),第6章組織化学SEM法" 医学出版センター, 399 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Yasunaga, A., Kato, S. Uchida, Y., Miyauchi, R.: "Enzyme-histochemical study on the fine distribution of the intramural lymphatics at the ileocecal junction of the monkey intestine." Okajimas Folia Anat. Jpn.68. 259-270 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kato, S., Yasunaga, A. Uchida, Y.: "Enzyme-histochemical method for identification of lymphatic capillaries." Lymphology. 24. 125-129 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Yasunaga, A., Uchida, Y. Kato, S.: "Enzyme-histochemical study of lymphatics and blood vessels in the lamina propria mucosae of the human large intestine. (in Japanese with English abstract)" Japanese Journal of lymphology. 14. 13-17 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kato, S., Yasunaga, A. Miyauchi, R.: "Enzyme-and immunohistochemical visualization of lymphatic capillaries in the mammalian thyroid gland:Light and electron microscopic study." Progress in Lymphology. XIII. 39-40 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kato, S., Yasunaga, A. Uchida, Y.: "Ul trastructural localization of 5'-nucleotidase in the lymphatic endothelium." Progress in Lymphology. XIII. 41-42 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kato, S., Yamada, A. Gotoh, M.: "Lymphatic imaging of the enzymehistochemical reaction by light and backscattered image scanning electron microscopy." Progress in Lymphology. XIII. 65-66 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Fujiwara, T., Kato, S. Torisu, T., Masumi, S.: "Enzyme-histochemical differentiation of lymphatic capillaries from blood capillaries in the synovial membrane of the human knee." Progress in Lymphology. XIII. 109-110 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Yasunaga, A., Uchida, Y. Kato, S., Masumi, S.: "Enzyme-histochemical study of the distribution of the lymphatics in human colorectal carcinoma." Progress in Lymphology. XIII. 397-399 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kato, S., Miura, M. Miyauchi, R.: "Enzyme-histochemical demonstration of lymphatic vessels in the subserosal layer of several organs by tissue spread method. (in Japanese with English abstract)" Japanese Journal of Lymphology. 16. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 加藤 征治,三浦 真弘,中村 英次郎,宮内 亮輔: "漿膜下組織内リンパ管網の酵素組織化学的描出-伸展試料の二重染色法" リンパ学. 16. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 加藤 征治(分担) 監修 宮澤 七郎/安達 公一: "医学・生物学の走査電子顕微鏡(分担)組織化学SEM法" 医学出版センター, 399 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] KATO,S.: "Enzymeーhistochemical identification of lymphatic vessels by light and backscattered image scanning electrom microscopy." Stain Technol.65. 131-137 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] KATO,S.: "Enzymeーhistochemical demonstration of lymphatic vessels in the mouse thymus." Arch.Histol.Cytol.35. 87-94 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] KATO,S.: "Histochemical localization of 5'ーnucleotidase in the lymphatic endothelium." Acta Histochem.Cytochem.23. 613-620 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 加藤 征治,安永 昭,内田 雄三,藤原 亨: "毛細リンパ管と毛細血管の酵素組織化学的鑑別法ー樹脂包埋(JBー4)を用いた5'ーnucleotidaseーalkaline phosphatase二重染色法" リンパ学. 13. 19-24 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] YASUNAGA,A.,KATO,S.UCHIDA,Y.and MIYAUCHI,R.: "Enzymeーhistochemical study on the fine distribution of the intramural lymphatics at the ileocecal junction of the monkey intestine." Okajumas Folia Anat.Jpn.68. 259-270 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] KATO.S.,YASUNAGA,A.and UCHIDA,Y.: "EnzymeーHistochemical method for identification of lymphatic capillaries." Lymphology. 24. 125-129 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 加藤 征治(分担)監修:宮沢 七郎,安達 公一: "医学・生物学の走査電子顕微鏡(基礎・応用・診断・アトラス)第6章組織化学SEM観察法" 医学出版センタ-(東京), 300 (1992)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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