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Activation Mechanism of Hepatocyte Growth Factor Sequestered in Extracellular matrix in liver

Research Project

Project/Area Number 03670016
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General anatomy (including Histology/Embryology)
Research InstitutionKitasato University

Principal Investigator

YAMAMOTO Noboru  北里大学, 看護学部, 教授 (10050543)

Co-Investigator(Kenkyū-buntansha) MASUMOTO Aki  北里大学, 看護学部, 助手 (30209457)
MATSUTANI Sinji  北里大学, 看護学部, 助手 (60219433)
HASEGAWA Takayuki  北里大学, 看護学部, 講師 (60180858)
Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1993: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1992: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1991: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsLatent HGF / latent HGF-activator / Activator of pro-HGF activator / liver injury / Extracellular Matrix / HGF / 肝細胞増殖促進 / 細胞外基質 / 肝再生 / bFGF / 核小体 / gene transcription制御 / 正常肝臓に存在する肝細胞増殖因子 / 癌細胞
Research Abstract

既に健常正獣ラット肝臓内に多量の潜在型HGFが存在することを明らかにした本年度はこの潜在型HGFの活性化機構を中心に検討を行った。
1)細胞外気質成分としてマトリゲルを用い、それにヒト胎盤から調製したHGF(プロトタイプ)を吸着させ、その培養基質上で肝細胞を培養すると著しい肝細胞DNA合成促進が認められた。
2)肝細胞を無血清培値で培養後、そのコンディションメジウム(CM)を回収した。そのCMを添加するとマトリゲル結合潜在型HGFの遊離・活性化が認められた。
これらの結果は肝細胞が細胞外基質に結合し、存在する潜在型HGFを活性化する因子を分泌することを示唆する。目下、この因子の精製・分析を行っている。

Report

(4 results)
  • 1993 Annual Research Report
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Aki Masumoto and Noboru Yamamoto: "Stimulation of DNA synthesis in Hepatocytes by Hepatocyte Growth Factor bound to Extracellular Matrix" Biochem.Biophys.Res.Comm. 191. 1218-1223 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Aki Masumoto and Noboru Yamamoto: "Characterization of a Hepatocyte Growth Factor Derived from Nonparenchymal Liver Cells" Cell Structure & Function. 18. 87-94 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Aki Masumoto and Noboru Yamamoto: "Stimulation of DNA synthesis in Hepatocytes by Hepatocyte Growth Factor bound to Extracellular Matrix" Biochem.Biophys.Res Comn.191. 1218-1223 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Aki Masumoto and Noboru Yamamoto: "Characterization of a Hepatocyte Growth Factor Devied from Nonparenchymal Liver Cells" Cell Structure & Function. 18. 87-94 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Noboru Yamanoto et al.: "Growth Stimulation of Adult Rat Hepatocyte in Primary Culture by Soluble Factor(s) Secreted from Nonparenchymal Liver Cell" Cell Struct.Funct.14. 217-229 (1989)

    • Related Report
      1992 Annual Research Report
  • [Publications] Shinji Matsutani et al.: "Morphometric analysis of stimulated changes in the distribution of secretory granules in subclone of PC12 cells." Biomed.Res.12. 347-350 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Aki Masumoto and Noboru Yamamoto: "Sequestration of a hepatocyte growth factor in extracellular matrix in normal adult rat liver" Biochem.Biophys.Res.Comm.174. 90-95 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] N.Yamamoto et al.: "Immunohistochemical localization of basic fibroblast growth factor in A431 human epidermoid carcinoma cells" Histochemistry. 96. 479-485 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] M.Wakakura and N.Yamamoto: "Rapid increase of intracellular Ca2+ concentration caused by aminoadipic acid enantiomers in retinal Muller cells and neuron in vitro" Documenta Opthalmologia. 80. 385-395 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Aki Masumoto and Noboru Yamamoto: "Stmulation of DNA synthesis in hepatocytes by hepatocyte growth factor bound to extracellular matrix" Biochem.Biophys.Res.Comm.176. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Aki Masumoto and Noboru Yamamoto:"SEQUESTRATION OF A HEPATOCYTE GROWTH FACTOR IN EXTRACELLULAR MATRIX IN NORMAL ADULT RAT LIVER." Biochem.Biophys.Res.Comm.174. 90-95 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] N.Yamamoto,S.Matsutani,Y.Yoshitake,K.Nishikawa:"IMMUNOHISTOCHEMICAL LOCALIZATION OF BASIC FIBROBLAST GROWTH FACTOR IN A431 HUMAN EPIDERMOID CARCINOMA CELLS." Histochemistry. 96. 479-485 (1991)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2017-05-19  

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