| Co-Investigator(Kenkyū-buntansha) |
NAKAYA Masayuki Jikei University, School of Medicine, Lecturer, 医学部医学科, 講師 (40056932)
TAKEUCHI Shuji Jikei University, School of Medicine, Lecturer, 医学部医学科, 講師 (40057055)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1992: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1991: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Research Abstract |
An atrophic phase of muscles was studied by using TS rat, suspended from the ceiling of cage. After 10 weeks, blood was taken under ether anaesthesia for a biochemical measurement. They were serum albumin, total cholesterol, urea nitrogen, creatinine, uric acid, CK, GOT, GPT and LDH. After that, some viscerals and muscles were removed and weighed. Some muscles were sectioned and stained by sudan black B for microscopic observation. Area of red and white muscle fibers was measured by automatic planimeter, which was connected to a computer.
The level of serum albumin and wet liver weight were significantly low in the TS group. It suggests the lowering of albumin, made in the liver, by 10weeks tail suspension. On the other side, the influence to muscle was calculated in next expression, i. e, (TS-C)/C X 100. The influenced degree was shown on the muscle weight, i. e, 12.7% in the tibialis anterior, 26.4% in the gastrocnemius and 23.4% in the plantaris. Those on the area of fibers were 6.6% of white and 3.7% of red fibers in the tibialis anterior, 23.7% of white and 17.9% of red in the gastrocnemius, 27.7% of white and 33.5% of red in the plantaris. These results suggest that the tail suspension strongly effects to the lower-limb flexors.
Considering other research articles, it will need to analyze various conditions. For example, microgravity, nutrients and exercise should be studied, in addition to the differences of age in experimental animals, or in each situated muscle.
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