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The dual orgin of trigeminal sensory neurons would correlate to the differences in nerve fiber distribution ?

Research Project

Project/Area Number 03670025
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 神経解剖学
Research InstitutionEhime University

Principal Investigator

KINUTANI Masae  Ehime Univ.Sch.Med., Ass.Prof., 医学部, 助教授 (60035491)

Co-Investigator(Kenkyū-buntansha) TAKASHIMA Yoichiro  Ehime Univ.Sch.Med., Prof., 医学部, 教授 (30028344)
TAKEUCHI Kyoko  Ehime Univ.Sch.Med., Instructor, 医学部, 助手 (80116954)
Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1993: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1992: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1991: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsPlacode / Chimera / Sensory ganglia / Trigeminal nerve / Transplantation / Development / Aves / プラコ-ド
Research Abstract

We designed to prepare the trigeminal ganglion chimera grafting the presumptive placode area between chicken and quail embryos. Using the chimera and MAb 39B11 which stains specifically the chick nerve fibers, peripheral nerve fibers of chimeric ganglion are able to distinguish their origins.
As preliminary experiments, normal trigeminal ganglion was observed histologically.
1) At stage 9.5, the otic placode began to thicken the epithelium. The placode for the Vth sensory ganglion, were first observed thickening or spurs at stages 11-12. The period of peak emigration from the placodes occurred at stages 14-17 and ceased to detach the cells from the epithelium at stages 18-19.
2) The quail presuptive placode areas of the Vth ganglion were grafted to chicken host at stage 9.5. Quail cells were the source of neurons located in the distal region of the trigeminal ganglion. Transplants of placodal material were occasionally contaminated with underlying mesectodermal mesenchymal cells, which were subsequently detected in branchial arches. This problem could not be dissolved by the careful mechanical dissection. By reason of the technical problem, we could not analyze the peripheral nerve fibers of chimeric ganglia.

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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