| Project/Area Number |
03670031
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
神経解剖学
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| Research Institution | St.Marianna University School of Medicine |
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Principal Investigator |
KANEKO Ritsuko St.Marianna University School of Medicine, Department of Anatomy, Lecturer, 医学部, 講師 (00161183)
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| Co-Investigator(Kenkyū-buntansha) |
HARA Masayuki St.Marianna University School of Medicine, Department of Anatomy, Lecturer, 医学部, 講師 (10198898)
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1992: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1991: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Ubiquitin / Cathepsin D / Proteolysis / Collateral elimination / Immunohistochemistry / Development / Rat |
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| Research Abstract |
Collateral elimination (CE) has been known to occur in corticospinal tract (CST) neurons during the postnatal development in rodents. However. how collterals eliminate remains unclear. Although some proteolytic processes might be involved in CE, few works have studied them. In the study, we focussed on ubiquitin and cathepsin-D. The former is a protein with many functions, one of which is related to the proteolysis, and the latter is the major protease in the lysosome. To examine their involvement in CE, we studied the ubiquitin- and cathepsin D-immunoreactive cells in the cervical cord in the pre- and postnatal rats.
Wistar-Imamichi rats at various pre- and postnatal stages, as well as adults, were used in this study. After the cervical cord with DRG was removed, it was fixed and embedded in paraffin for LM or embedded in Epon for EM. The sections were stained immunohistochemically, using ubiquitin- or cathepsin D-antiserum. We found numerous cathepsin D-immunoreactive cells in and near CST during the development, suggesting its involvement in CE of CST neurons. Although clear ubiquitin-immunoreactivity could not be observed in CST, we found ubiquitin-immunoreactive nuclei in motoneurons and ganglion cells in the DRG. Ubiquitin might have some other functions than proteolysis in the nucleus of developing neurons.
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