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Role of gap-junctional communication in cell proliferation and adipocyte conversion

Research Project

Project/Area Number 03670038
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General physiology
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

SHIBA Yoshiki  School of Dentistry, Department of Physiology, Associate Professor, 歯学部, 助教授 (90110452)

Co-Investigator(Kenkyū-buntansha) HIRONO Chikara  School of Dentistry, Department of Physiology, Research Associate, 歯学部, 助手 (10199135)
SUGITA Makoto  School of Dentistry, Department of Physiology, Research Associate, 歯学部, 助手 (50235884)
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1992: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1991: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsCell communication / Cell proliferation / Cell differentiation / Adipocytes / Fibronectin / 脂肪細胞
Research Abstract

Gap junctional intercellular communication is considered to play an important role in the control of cell proliferation and cell differentiation, and the exact mechanism of its control was investigated. Growth Stimulation by the serum transiently suppressed the gap-junctional communication and then enhanced DNA synthesis in 3t3-L1 cells. Pretreatment of quiescent cells with 12-O-tetradecanoylphorbol-13-acetate (TPA) suppressed the gap-junctional communication and enhanced serum-induced DNA synthesis. Pretreatment with dibutyryl cyclic AMP (cAMP) stimulated the cell communication and suppressed serum-induced DNA synthesis. The addition of the serum induced adipocyte conversion from 3T3-L1 cells. TPA inhibited adipocyte conversion and cAMP enhanced adipocyte conversion. Substances exchanged through gap junctions were investigated by fractionating the cell homoginates. Calcium ions are candidates for small substances permeable through gap junction.s Growth stimulation also induced the increase in the intracellular concentration of calcium ions. Treatment with TPA also enhanced the increase in calcium ions and cAMP suppressed it. In this project, the gap-junctional communication is suggested to be involved in the control of cell proliferation and cell differentiation.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] 柴 芳樹: "Localization of fibronectin receptors and its modulation by growth stimulation" In Vitro. 27. 62A-62A (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 柴 芳樹: "Regulation of gap junctional intercellular communication and control of cell growth" Cell Struct.Funct.16. 525-525 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 柴 芳樹: "ギャップの結合の機能" 細胞. 24. 394-398 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 柴 芳樹: "Anchorage-dependent protection from cell death by epidermal growth factor from salivary gland" Dentistry in Japan. 29. 51-54 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 柴 芳樹: "細胞集団の機能調節" 動物たちの地球. 84. 211-215 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shiba, Y.: "Localization of fibronectin receptors and its modulation by growth stimulation" In Vitro. 27. 62A-62A (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shiba, Y.: "Regulation of gap junctional intercellular communication and control of cell growth" Cell Struct. Funct.16. 525-525 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shiba, Y.: "Anchorage-dependent protection from cell death by epidermal growth factor from salivary gland" Dentistry in Japan. 29. 51-54 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shiba, Y.: "saibou shundann no kinou chousetu" Doubutu tachi no chikyu. 84. 211-215 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 柴 芳樹: "ギャップ結合の機能" 細胞. 24. 394-398 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 柴 芳樹: "細胞集団の機能調節" 動物たちの地球. 84. 211-215 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 柴 芳樹: "Anchorage-dependent protection from cell death by epidermal growth factor from salivary gland" Dentistry in Japan. 29. 51-54 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Shiba,Y.: "Localization of fibronectin receptors and its modulation by growth stmulation." In Vitro. 27. 62A (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Shiba,Y.: "Regulation of gap junctional intercellular clmmunication and control of cell growth" Cell Struct.Funct.16. 525 (1991)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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