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Changes in lactotroph responsiveness to secretagogues as determined by the sequential cell immunoblot assay

Research Project

Project/Area Number 03670081
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 環境生理学(含体力医学・栄養生理学)
Research InstitutionYamanashi Medical University (1993)
Yokohama City University (1991-1992)

Principal Investigator

ARITA Jun  Yamanashi Medical University Professor, 医学部, 教授 (80128587)

Co-Investigator(Kenkyū-buntansha) ENDO Yutaka  Yokohama City Univ., Sch.Med., Instructor, 医学部, 助手 (90194050)
Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1993: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1992: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1991: ¥900,000 (Direct Cost: ¥900,000)
KeywordsAnterior pituitary gland / Prolactin / Dopamine / TRH / Estrogen / Sequential cell immunoblot assay / 甲状腺刺激ホルモン放出ホルモン / ド-パミン
Research Abstract

We investigated heterogeneity for the responsiveness of lactotrophs to secretagogues and the effects of estradiol on the responsiveness by quantifying prolactin secretion at the single cell level.
1. Heterogeneity for the responsiveness of lactotrophs to thyrotropin-releasing hormone(TRH)
(1) Although prolactin secretion from single lactotrophs in culture was increased by TRH in a dose-dependent manner, approximately 50% of total lactotrophs were not stimulated by TRH even at a maximally effective dose. This result suggests that there are two subpopulations of lactotrophs that are responsive and unresponsive to TRH.
(2) A population of lactotrophs that secrete small amounts of prolactin under basal conditions contains a much larger proportion of TRH-responsive lactotrophs than does a population of lactotrophs that secrete large amounts of prolactin.
2. Changes by estradio1 in the responsiveness of lactotrophs to secretagogues
(1) When the effects of estradiol treatment on prolactin secretion from a population of lactotrophs to medium were examined, it was found that estradiol increased basal secretion of prolactin. Estradiol attenuated and enhanced the inhibitory action of dopamine and the stimulatory action of TRH, respectively, on prolactin secretion.

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] J.ARITA: "Lactotrophs Secreting Small Amounts of Prolactin Reveal Great Responsiveness to Thyrotropin-Releasing Hormone:Analysis by the Sequential Cell Immunoblot Assay" Endocrinology. 130. 3167-3174 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] J.ARITA: "Heterogeneity in the Secretory Activity of Pituitary Lactotrophs Revealed by Analysis at the Single Cell Level" Yokohama Medical Bulletin. 43. 61-80 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] J.ARITA: "Analysis of the Secretion from Single Anterior Pituitary Cells by Cell Immunoblot Assay" Endocrine Journal. 40. 1-15 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] JUN ARITA, YASUHIRO KOJIMA, FUKUKO KIMURA: "Lactotrophs secreting small amounts of prolactin reveal great responsiveness to thyrotropin-releasing hormone : analysis by the sequential cell immunoblot assay" Endocrinology. 130. 3167-3174 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] JUN ARITA: "Analysis of the secretion from single anterior pituitary cells by cell immunoblot assay" Endocrine Journal. 40. 1-15 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] JUN ARITA: "Heterogeneity in the secretory activity of pituitary lactotrophs revealed by analysis at the single cell level" Yokohama Med.Bull. 43. 61-80 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] J.ARITA: "Lactotrophs secreting small amounts of prolactin reveal great responsiveness to thyrotropin-releasing hormone" ENDOCRINOLOGY. 130. 3167-3174 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] J.ARITA: "Analysis of the secretion from single anterior pituitary cells by cell immunoblot assay" ENDOCRINE JOURNAL. 40. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] J.ARITA: "Heterogeneity in the secretory activity of pitutitary lactotrophs revealed by analysis at the single cell level" YOKOHAMA MEDICAL BULLETIN. 43. (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] J.ARITA: "Lactotrophs secreting small amounts of prolactin reveal great responsiveness to thyrotropinーreleasing hormone" ENDOCRINOLOGY. 130. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] J.ARITA: "Heterogeneity in the secretory activity of pituitary lactotrophs revealed by analysis at the single cell level" YOKOHAMA MEDICAL BULLETIN. (1992)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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