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Long term potentiation of paintransmission at the spinal level

Research Project

Project/Area Number 03670096
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General pharmacology
Research InstitutionTokyo Medical and Dental University

Principal Investigator

YOSHIOKA Koichi  Faculty of Medicine, Tokyo Medical and Dental University, Associate Professor, 医学部, 助教授 (00143579)

Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1992: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1991: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywordssubstance P / tachykinins / spinal cord / pain / serotonin / アセチルコリン / 鎮痛 / ムスカリン性受容体
Research Abstract

1. Transmitter mechanisms of a long-lasting inhibition of the monosynaptic reflex (MSR)induced by stimulation of the descending pathway was investigated in the isolated spinal cord of the neonatal rat. The descending inhibition was markedly potentiated by a 5-hydroxytryptamine (5-HT) uptake blocker and was blocked by a 5-HT antagonist, suggesting the involvement of 5-HT in this inhibition.
2. The mechanisms of a cutaneous nerve-evoked inhibition of MSR were studied in the spinal cord-peripheral nerve preparation. Conditioning stimulation of the saphenous nerve evoked an inhibition of the MSR. This inhibition was potentiated by an anticholinesterase and almost completely blocked by atropine. From the effects of muscarinic agonists and antagonists, it was suggested that the receptors involved in the inhibition of MSR are of M2 type.
3. A possible mechanism of inactivation of tachykinins released from nerve terminals is enzymatic degradation. To investigate this possibility, effect of peptidase inhibitors on C-fiber evoked responses was examined using an isolated spinal cord-saphenous nerve preparation of the newborn rat. A slow depolarization of the L3 ventral root evoked by the saphenous nerve stimulation was enhanced by application of a mixture of peptidase inhibitors in the presence of naloxone. This result suggests that enzymatic degradation plays a physiological role in termination of neurotransmitter action of tachykinins.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] YOMONO H.S.et al.: "Serotonergic fibers induce a long-lasting inhibition of monosynaptic reflex in the neonatal rat spinal cord." Neuroscience. 47. 521-531 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] YANAGISAWA M.et al.: "Enzymatic inactivation of tachykinin neurotransmitters in the isolated spinal cord of the newborn rat." Neurosci.Res.15. 289-292 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] SUZUKI H.et al.: "Potentiating effect of peptidase inhibitors on a C fiber-evoked response in the isolated spinal cord preparation of the neonatal rat." Regul.Pept.Suppl.1. S152 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] MAEHARA T.et al.: "Substance P-eboked release of aminoacid transmitters from the newborn rat spinal cord." Regul.Pept.Suppl.1. S102 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] SUZUKI H.et al.: "Peptidase inhibitors potentiate a cutaneous nerve-evoked slow depolarization in the isolated spinal cord of the neonatal rat." Neurosci.Res.Suppl.17. S118 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] MAEHARA.T.et al.: "Effect of sodium and chloride ions on substance P-induced release of GABA from the neonatal rat spinal cord." Neurosci.Res.Suppl.17. S117 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Sakuma M.: "Substance P-evoked release of GABA from isolated spinal cord of the newborn rat." Neuroscience. 45. 323-330 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kobayashi N.: "Substance P-evoked release of acetylcholine from isolated spinal cord of the newborn rat." Neuroscience. 45. 330-337 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Yoshioka K.: "Cutaneous nerve-evoked cholinergic inhibition of monosynaptic reflex in the neonatal rat spinal cord." Neuroscience. 38. 195-203 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Yomono H.S.et al.: "Serotonergic fibers induce a long-Lasting inhibition of monosynaptic reflex in the neonatal rat spinal cord." Neuroscience. 47. 521-531 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Yanagisawa M.et al.: "Enzymatic inactivation of tachykinin neurotransmitters in the isolated spinal cord of the newborn rat." Neurosci.Res.15. 289-292 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Suzuki H.et al.: "Potentiating effect of peptidase inhibitors on a C fiber-evoked response in the isolated spinal cord preparation of the neonatal rat." Regul.Pept.Suppl.1. S152- (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Maehara T.et al.: "Substance P-evoked release of amino acid transmitters from the newborn rat spinal cord." Regul.Pept.Suppl.1. S102- (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Suzuki H.et al.: "Peptidase inhibitors potentiate a cutaneous nerve-evoked slow depolarization in the isolated spinal cord of the neonatal rat." Neurosci.Res.Suppl.17. S118- (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Maehara T.et al.: "Effect of sodium and chloride ions on substance P-induced release of GABA from the neonatal rat spinal cord." Neurosci.Res.Suppl.17. S117- (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] M.Sakuma: "Substance Pーevoked release of GABA from isolated spinal cord of the newborn rat." Neuroscience. 45. 323-330 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] N.Kobayashi: "Substance Pーevoked release of acetylcholine from isolated spinal cord of the newborn rat." Neuroscience. 45. 331-337 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] M.Otsuka: "Tachykininーevoked release ofneurotrar smitters from isolated spinal cord of the newborn rat." Annals of the New York Academy of Sciences. 632. 212-219 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Kurihara: "Effects of opioids on the slow inhibition of monosynaptic reflex in the newborn rat spinal cord." Neuroscience Research. Suppl.14. S114 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] K.Yoshioka: "Facilitatory and inhibitory muscarinic modulation of C fiberーevoked depolarization in the neonatal rat spinal cord." Neuroscience Research. Suppl.14. S134 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] H.Suzuki: "Characteristics of tachykinin receptors mediating acetylcholine release from the neonatal rat spinal cord." Neuroscience Research. Suppl.14. S150 (1991)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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