| Project/Area Number |
03670194
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
寄生虫学(含医用動物学）
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| Research Institution | Kochi Medical School |
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Principal Investigator |
AGATSUMA Takeshi Kochi Medical School, Research Associate, 医学部, 助手 (40117031)
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1992: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1991: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | Schistosoma mansoni / Female-specific gene / Chorion gene / fs 800 gene / fs800 / 日本住血吸虫 / DNA / 卵殻タンパク遺伝子 / クロ-ニング |
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| Research Abstract |
Egg production of worm pairs is a major cause of pathogenesis in schistosomiasis. Thus an understanding of female reproductive development, especially mechanisms of female-specific gene regulation is of major importance in disease control. A previously isolated cDNA that encodes the fs 800 protein was used to identify and isolate a recombinant phage containing an entire copy of the fs 800 gene. The gene is contained on a 5.5 kbp EcoRI fragment. There are no introns. The cap-site of the mRNA was determined by primer extension using RNA as template and dideoxy sequencing. The upstream regions of the gene contain putative cis-acting elements (TCACGT, GTAGAAT) that have been shown to play a role in the regulation of chorion gene expression in insects. The other elements that share a common core motif, TRRAAT(R=pyrimidine), are also found. These elements have been shown to bind nuclear proteins and control quantitative and qualitative changes in reporter gene expression in silkmoths.
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