Biochemical studies on aerobic-anaerobic respiratory transition in mitochondria from parasitic helminthes
| Project/Area Number |
03670201
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
寄生虫学(含医用動物学）
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| Research Institution | Juntendo University School of Medicine |
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Principal Investigator |
TAKAMIYA Shinzaburo Parasitology Associate professor, 医学部, 助教授 (90138206)
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| Co-Investigator(Kenkyū-buntansha) |
FURUSHIMA Rieko Parasitology Research associate, 医学部, 助手 (50146776)
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1992: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1991: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Ascaris mitochondria / complex II / respiratory conversion / Paragonimus mito / ubiquinone / rhodoquinone / anaerobic respiration / aerobic respiration |
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| Research Abstract |
1. The primary structures of the Ip subunit of complex II of adult Ascaris were determined using peptides separated on Biogel P-60 in the presence of sodium dodecylsulfate. The alanine-threonine cluster, a conserved region in the amino terminus of the mammalian Ip subunit, was found in the Ascaris Ip subunit. Two of the three cystein-rich segments generally conserved in the Ip subunit were sequenced following chemical modification of cystein residues. From a comparative analysis of the sequences, the Ip subunit of complex II in Ascaris adult appears to be more closely related to those of bacterial SDH and mammalian complex II than to that of bacterial FRD, even though Ascaris complex II shows a high FRD activity.
2. To elucidate mechanisms of aerobic-anaerobic respiratory transition during development of Ascaris nematode, the larval respiratory chain and its complex II have been characterized in the isolated mitochondria by spectrophotometry, immunoblotting using anti adult complex II antibodies and determination of quinones. The results obtained showed that L2 larvae were the most aerobic among stages studied and the larval complex II was different from the adult counterpart. Furthermore, evidence showing biosynthetic conversion of quinones durig development was obtained.
3. Mitochondria from a lung fluke, Paragonimus westermani, were isolated to study the worm respiratory chain. Interestingly, the adult worm mitochondria were shown to possess both aerobic and anaerobic respiratory chains, i. e., cyanide-sensitive succinate oxidase and NADH-fumarate reductase system, respectively. The mitochondria were also found to contain both rhodoquinone-10 and ubiquinone-10.
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Report
(3 results)
Research Products
(8 results)
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[Publications] Takamiya, S., Kita, K., Wang, H., Weinstein, P.P., Hiraishi, A., Oya, H. and Aoki, T.: "Developmental changes in the respiratory chain of Ascaris mitochondria" Biochim. Biophys. Acta. 1141. 65-74 (1993)
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