• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of the mechanism of the invasion of the A subunit of heat-labile enterotoxin from enterotoxigenic E coli

Research Project

Project/Area Number 03670225
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 細菌学
Research InstitutionFujita Health University

Principal Investigator

TSUJI Takao  Fujita Health Univ., Medicine, Associate Professor, 医学部, 助教授 (60171998)

Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1992: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1991: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywordsenterotoxigenic / Escherichia coli / heat-labile-enterotoxin / nick / ADP-ribosylation / site direct mutagenesis / alosteric effect / 易熱性エンデドキシン / コレラ菌 / A,Bサブユニット / 変異株
Research Abstract

In this time, we can get the data as followed ;
1. Vibrio cholerae produced the protease(this is named as VC-1) that could nick at the position 192(Arg) from the N-terminus of the A subunit of unnicked heat-labile enterotoxins from enterotoxigenic Escherichia coli.
2. The Chinese hamster overly cell, which is elongated with the addition of toxin to its culture medium, produced the protease that could nick the unnicked heat-labile enterotoxin A subunit.
3. THE free A subunit, which did not bind to B subunit, was detected in the cell extract from enterotoxigenic E.coli and was purified. It was very unstable and its biological activity was lower than that of the normal A subunit.
4. There was the heterogeneity among the heat-labile enterotoxins(LTp) purified from the porcine enterotoxigenic E.coli strains. 5. The amino acid substitution at the position 112 of the A subunit from Glu to Lys(E112X) affects the ADP-ribosyltransferase activity of its A1 flagment. Therefore it is suggested that Glu 112 is important for the its ADP-ribosyltransferase activity.
6. Though the E112X mutant LT did not have ADP-ribosyltransferase activity, it had the inhibition activity of the interaction between ADP ribosylation factor(ARF) to cholera toxin. These data indicates that the ARF could activate the cholera toxin with the alosteric effect.
7. My coworker, Dr.Shida, found that there were two kinds of the glycoprotein, which could react with LT, in heated cow milk, but not in unheated one. By amino acid analyze of them, they were alpha-lactoalbumin and beta-lactoglobulin.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] Joel moss et al: "Interaction of ADP-ribosylation factor with Escherichia coli enterotoxin that contain an inactivating Lysine 112 substitution" Journal of Biological Chemistry. (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Takashi INOUE et al: "Amino acid sequence of heat-labile enterotoxin from chicken enterotoxigenic Escherichia coli is identical to that of human H10407" FEMS.Microbiol.Lett.(1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kan SHIDA et al: "Escherichia coli heat-labile enterotoxin binding glycoprotein in a proteose-peptone fraction of bovine milk" Journal of Daily Science. (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Noriko AKASHI et al: "Production of heat-labile enterotoxinII from chicken clinical isolates of Escherichia coli" FEMS.Microbiol.Lett.(1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Nils Lycke et al: "The adjuvant effect of Vibrio cholerae and Escherichia coli heat-labile enterotoxins is linked to their ADP-ribosyltransferase activity." Eur.J.Eqidemiol.22. 2277-2281 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Takao TSUJI et al: "Heterogeneity among heat-labile enterotoxins produced by porcine enterotoxigenic Escherichia coli" Eur.J.Equidemiol.8. 543-547 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 辻 孝雄: "毒素原性大腸菌の発生する易熱性エントロテキシン(LT)の活性部位" 日本細菌学雑誌, 16 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 辻 孝雄: "毒素原性大腸菌の発生する易熱性エントロテキシンの活性部位" 日本生化学会, 5 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Moss.J et al: "Interaction of ADP-ribosylation factor with E.coli enterotoxins that contain an inactivating Lysine 112 substitution" J.Biol.Chem. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Inoue T et al: "Amino acid sequence of heat-labile enterotoxin from chicken enterotoxigenic E.coli is identical to that of human H10407 strain" FEMS.Microbiol.Lett. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shida K et al: "E.coli heat-labile enterotoxin binding glycoprotein in a proteose-peptone fraction of bovine milk" J.Dairy.Sci. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Akashi N et al: "Production of heat-stable enterotoxin II from chicken clinical isolates of E coli" FEMS.Microbiol.Lett. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Lycke N et al: "The adjutant effect of Vibrio cholerae and E.coli heat-labile enterotoxins is linked to their ADP-ribosyltransferase activity" Eur.J.Immunol. 22. 2277-2281 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Takao Tsuji et al: "Heterogeneity among heat-labile enterotoxins produced by porcine enterotoxigenic E.coli" Eur.J.Epidemiol. 8. 543-547 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Joel Moss, Takao Tsuji et al: "Interaction of ADP-ribosylation factor with Escherichia coli enterotoxin that contain an inactivating Lysine 112 substitution" Journal of Biological Chemistry. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Takashi Inoue,Takao Tsuji et al.: "Amino acid sequence of heat-labile enterotoxin from chicken enterotoxigenic Escherichia coli is identical to that of human H10407" FEMS Microbiol.Letters. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Nils Lycke,Takao Tsuji et al.: "The adjuvant of V.cholerae and E.coli heat-labile enterotoxins is linked to their ADP-ribosyltransferase activity." Eur.J.Immunol.22. 2277-2281 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Y.Ichinose,T Tsuji et al: "The protease from V.cholerae nicks argine at position 192 from the N-terminus of the heat-labile enterotoxin A subunit from enterotoxigenic E.coli" Eur.J.Epiderm.8. 743-747 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Takao Tsuji and Akio Miyama: "Heterogeneity among heat-labile enterotoxins produced by porcine enterotoxigenic Escherichia coli" Eur.J.Epiderm.8. 543-547 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Takao Tsuji,and Akio Miyama: "Chinese hamster overy cells produced an enzyme that nicks heat-labile enteotoxins from enterotoxigenic Escherichia coli" Eur.J.Epiderm. 8. 74-80 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 辻 孝雄: "毒素原性大腸菌の産生する易熱性エンテロトキシン(LT)の活性部位" 日本細菌学雑誌, 16 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 辻 孝雄: "毒素原性大腸菌の産生する易熱性エンテロトキシンの活性部位" 日本生化学会(生化学), 5 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Takao Tsuji et al: "Dettction and purification of the free A subunit of heat-labile enterotoxin produced by enterotoxigenic Escherichia coli" FEMS・Microbiol・Lett・. 77. 277-282 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kiichi Imamura et al: "Purification of ornithine decarboxylase-inducing factor from cell-free ascites fluid of Ehrlich ascites tumor and its characteristics" Jpn.J.Cancer.Res.82. 315-324 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Takao Tsuji et al: "Glutamic acid-112 of the A subunit of heat-labile enterotoxin from enterotoxigenic Escherichia coli is important for ADP-ribosyltransferase activity" FEBS.Lett.291. 319-321 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Takao Tsuji et al: "Chinese hamster overy cells produce an enzyme that nicks heat-labile enterotoxin from enterotoxigenic Escherichia coli" European.J.Epidemiology. 8. 74-80 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Takao Tsuji et al: "Heterogeneity of the heat-labile enterotoxin from porcine enterotoxigenic Escherichia coli" European.J.Epidemiology.(1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Ichinose,S et al: "The protease from V.cholerae nicks arginie at position 192 form the N-terminus of the heat-labile enterotoxin A subunit from enterotoxigenic Escherichia coli" European.J.Epidemiology.(1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 辻 孝雄,福山 恵一: "生化学" 日本生化学会,

    • Related Report
      1991 Annual Research Report

URL: 

Published: 1991-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi