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Detection of amino acid motifs on the agretopes of antigens highly bound to MHC molecules

Research Project

Project/Area Number 03670243
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Immunology
Research InstitutionHokkaido University

Principal Investigator

OGASAWARA Kazumasa  Hokkaido Univ. Inst. of lmmunol. sci.,, 免疫科学研究所, 助教授 (20169163)

Co-Investigator(Kenkyū-buntansha) IWABUCHI Kazuya  Hokkaido Univ. Inst. of lmmunol. sci.,, 免疫科学研究所, 助手 (20184898)
ONOE Kazunori  Hokkaido Univ. Inst. of lmmunol. sci.,, 免疫科学研究所, 教授 (40002117)
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1992: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1991: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywordstri-molecular complex / agretope / epitope / DQw6 transgenic mice / haemagglutinin / cassette theory / アグレト-プ / エピト-プ / カセットセオリ- / ペプチドワクチン
Research Abstract

After constitution of tri-molecular complexes among TCR, MHC molecules,and peptide fragments of antigens, T cells are activated. In this event, binding between antigen peptides and MHC molecules is critical for constitution of the tri-molecular complex. we have reported pigeon cytochrome c related peptides substituted at agretopes (sites bound to MHC molecules) bound to murine MHC class II molecules in an allele specific manner. In order to detect peptides bound to human class II molecules we tried to establish DR transgenic mice. Since we detected the injected DNAs in some mice by PCR technique, we are testing expression of the DR molecules in various tissues of the mice.In another work we found that pigeon cytochrome c related peptides bound to DQw6 molecules and activated T cells in the DQw6 transgenic mice kindly provided by Dr. Sasazuki. Peptides carrying arginine at position 46 and alanine at position 54 which had been determined as agretopes bound to DQw6 molecules (AEGRSYTVANKAKGIT). Further, we found a peptide consisted of influenza virus haemagglutinin 123-138 (EGFTWTGVTQNGGSNA) bound to DQw6 molecules.
We have also reported that pigeon cytochrome c related peptides carrying the murine MHC binding motif with substituted epitopic residues (sites bound to TCR) to haemagglutinin of influenza virus can stimulate immune responses to influenza virus in mice (cassette theory). Since the motif bound to human MHC molecules has been appeared, peptide vaccines prepared by the cassette theory to influenza virus will be effective in human.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] GOTOHDA,T: "Analysis of functional sites on a peptide antigen,p43-58,in I-A or I-E-restricted T cell responses." International Immunology. 3. 503-509 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] NEGISHI,I.: "H-2K molecules positively select V17βa^+CD4^-8^+T cells in bone marrow and thymic chimeras." Cellular Immunology. 136. 185-193 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] ITOH,Y.: "A hole in the T cell repertoire specific for a pigeon cytochrome crelated peptide associated with amono acid substitutions on-A^b molecules." International Immunology. 4. 779-787 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] OGASAWARA,K: "A strategy for making synthetic peptide vaccines." Proc.Natl.Acad.Sci.USA. 89. 8995-8999 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 小笠原 一誠: "MHCとポリペプチドーワクチンのデザイン-" Medical Immunology. 22. 841-848 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 小笠原 一誠: "MHC分子とペプチド結合" 実験医学. 10. 561-563 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 小笠原 一誠(分担): "Anual Review細胞生物学1991" 中外医学社, 395 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 小笠原 一誠(分担): "新免疫学読本" 日本評論社, 208 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Gotohda,T.: "Analysis of functional sites on a peptide antigen, p43-58, in I-A or I-E-restricted T cell responses." Int. Immunol.3. 503-509 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Negishi,I.: "H-2K molecules positively select V17beta alpha^+CD4^-8^+T cells in bone marrow and thymic chimeras." Cell. Immunol.136. 185-193 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Itoh,Y.: "A hole in T cell repertoire specific for a pigeon cytochrome c related peptide in bm12 mice." Int. Immunol.4. 779-787 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Ogasawara,K.: "A strategy for making synthetic peptide vaccines." Proc. Natl. Acad. Sci. USA. 89. 8995-8999 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Ogasawara,K.: "MHC and polypeptide-design of vaccines-" Med. Immunol.22. 841-848 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Ogasawara,K.: "Binding between MHC molecules and peptide." Exp. Med.10. 561-563 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Ogasawara,K. (partial charge): Ann. Rev. Cell Biol. 1991, 395 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Ogasawara,K. (partial charge): New immunol. Read., 208 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Gotohda,T.: "Analysis of functional sites on a peptide antigen,p43-58,in I-A or I-E-restricted T cell responses." International Immunology. 3. 503-509 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Negishi,I.: "H-2K molecules positively select V17βa^+CD4^-8^+T cells in bone marrow and thymic chimeras." Cellular Immunology. 136. 185-193 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Itoh,Y.: "A hole in the T cell repertoire specific for a pigeon cytochrome c related peptide associated with amino acid substitutions on I-A^b molecules." International Immunology. 4. 779-787 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Ogasawara,K.: "A strategy for making synthetic peptide vaccines." Proc.Natl.Acad.Sci.USA. 89. 8995-8999 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 小笠原 一誠: "MHCとポリペプチドーワクチンのデザインー" Medical Immunology. 22. 841-848 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] 小笠原 一誠: "MHC分子とペプチド結合" 実験医学. 10. 561-563 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 小笠原 一誠(分担): "Anual Review 細胞生物学1991" 中外医学社, 395 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] 小笠原 一誠(分担): "新免疫学読本" 日本評論社, 208 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Gotohda,Toshihiko: "Analysis of functional sites on a peptide antigen,p43ー58,in IーA or IーEーrestricted T cell responses" International Immunology. 3. 503-509 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Itoh,Yasushi: "A hole in T cell repertoire specific for a pigeon cytochrome c rerated peptide in bm12 mice."

    • Related Report
      1991 Annual Research Report
  • [Publications] Itoh,Yasushi: "Detection of IーA allele specific amino acids on the agretopes of the pigeon cytochrome c related peptides."

    • Related Report
      1991 Annual Research Report
  • [Publications] 小笠原 一誠: "T細胞レパ-トリ-に欠損部分ができるか" 医学のあゆみ. 157. 634 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 小笠原 一誠: "MHCとポリペプチドワクチンのデザイン" Medical Immunology. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 小笠原 一誠: "MHC分子とペプチド結合" 実験医学. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 小笠原 一誠(分担): "Annual Review細胞生物学1991" 中外医学社, 395 (1991)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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