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A study on the cell interaction between stromal cells and lymphocytes

Research Project

Project/Area Number 03670246
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Immunology
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

KINA Tatsuo  Chest Disease Research Institute, Kyoto University Associate Professor, 胸部疾患研究所, 助教授 (30127071)

Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1992: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1991: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsStromal cell / Adhesion molecule / Cell interaction / Lymphocytes / VCAM-1 / VLA-4 / ストロ-マ細胞 / モノクロ-ナル抗体 / インテグリン分子 / サイトカイン / Stem Cell Factor / MーCSF
Research Abstract

Function of cell surface 107kD glycoprotein(gp107) of stromal cells in lymphocyte development was investigated We have established two monoclonal antibodies (HANA-2 and HANA-5) against 107kD glycoprotein(s),and studied the expression and function of gp107 in hematopoietic system. In normal hematopoietic system, HANA-2 reacts only with fibroblastoid cells in the reticuloendothelia system, but not with lymphoid, myeloid and epithelial cells. On the other hand, HAHAN-5 reacts with activated lymphocytes and myeloid cells as well as established stromal cells. In the in vitro hematopoietic system, although HANA-5 strongly inhibited the development of both lymphoid and myeloid cells, HANA-2 inhibited only lymphoid development. HANA-2 but not HANA-5 could block the binding of lymphocytes with stromal cells, suggesting that HANA-2 reacts with an adhesion molecule on the stromal cells. By isolating cDNA clones for HANA-2 antigen, we identified it as an moue homologue of human vascular cell adhesion molecule, VCAM-1. This was further confirmed by the findings that antibodies against mouse VLA-4 strongly blocked lymphocye adhesion to stromal cells. HANA-5 has no blocking effects on cell adhesion and seems to be an activated antigen of both hamatopoietic and stromal cells, These results suggest an important role of VLA-4/VCAM-1 interaction in the development of lymphoid cells and that 107kD poteins on the stromal cell surface consist of at least two different molecules, one of which is VCAM-1.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Kina,T.: "Idenfification of a 107-kD glycoprotein that mediates adhesion between stromal cells and hematolymphoid cells." Journal of Experimental Medicine. 173. 373-381 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Ogawa,M: "Expression and function of c-kit in hemopoietic progenitor cells." Journal of Experimental Medicine. 174. 63-71 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Ikuta,K.: "Development of gd T-cell subsets from fetal hematopoietic stem cells." Annals of New York Academy of Sciences. 651. 21-32 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Ogawa,M: "Expression and function of c-Kit in fetal hemopoietic progenitor cells" Development. 117. (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 喜納 辰夫: "胸腺間質細胞とTリンパ球との相互作用" Immunoheamatology. 12. 199-204 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 井手 山晋: "胸腺ストローマ細胞-その特徴と役割" 臨床免疫. 24. 399-407 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 喜納 辰夫(共著): "接着分子" メジカルビュー社, 175 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 喜納 辰夫(共著): "Annual Review 免疫1992" 中外医学社, 289 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kina, T., Sen Majumdar, A., Heimfeld, S., Kaneshima, H., Holzmann, B., Katsura, Y., and Weissman, I. L.: "Identification of a 107-kD glycoprotein that mediates adhesion between stromal cells and hematolymphoid cells." J. Exp. Med.173. 373-381 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Ogawa, M., Matsuzaki, Y., Nishikiwa, S., Hayashi,S-I., Kunisada, T., Sudo, T., Kina, T., Nakauchi, H. and Nishikawa S-I.: "Expression and function of c-kit in hemopoietic progenitor cells." J. Exp. Med.174. 63-71 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Ikuta, K., Kina, T., MacNeil, I., Uchida, N., Peault, B., Chien, Y. and Weissman, I. L.: "Development of gammadelta T-cell subsets from fetal hematopoietic stem cells." Ann. N. Y. Acad. Sci.651. 21-32 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Ogawa, M., Nishikawa, S., Yoshinaga, K., Hayashi, S-I., Kunisada, T., Nakao, J., Kina, T., Sudo, T., Kodama, H., and Nishikawa, S-I.: "Expression and function of c-Kit in fetal hemopoietic progenitor cells: transition from the early c-kit-independent to the late c-kit-dependent wave of hemopoiesisin the murin embryo." Development.117. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kina,T.: "Idenfification of a 107-KD glycoprotein that mediates adhesion between stromal cells and hematolymphoid cells." Journal of Experimental Medicine. 173. 373-381 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Ogawa,M: "Expression and function of c-kit in hemopoietic progenitor cells." Journal of Experimental Medicine. 174. 63-71 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Ikuta,K.: "Development of gd T-cell subsets from fetal hematopoietic stem cells." Annals of New York Academy of Sciences. 651. 21-32 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Ogawa,M: "Expression and function of c-Kit in fetal hemopoietic progenitor cells" Development.117. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 喜納 辰夫: "胸腺間質細胞とTリンパ球との相互作用" Immunoheamatology. 12. 199-204 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] 井手山 晋: "胸腺ストローマ細胞-その特徴と役割" 臨床免疫. 24. 399-407 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 喜納 辰夫(共著): "接着分子" メジカルビュー社, 175 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] 喜納 辰夫(共著): "Annual Review免疫1992" 中外医学社, 289 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kina,T.: "Identification of a 107KD glycoprotein that mediates adhesion between stromal cells and hematolymphoid cells" J.Exgerimental Medicine. 173. 373-381 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Ogawa,M.: "Expression and Function of Cーkit in Hemopoietic Progenitor cells" J.Exp.Med.174. 63-71 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Ikuta,K.: "Development of rs Tーcell subsets from fetal hematopoietic stem cells" Ann.N.Y.Acad.Sci.

    • Related Report
      1991 Annual Research Report
  • [Publications] 宮坂 昌之編集: "接着分子" メジカルビュ-社, 175 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 喜納 辰夫,他: "Annual Review免疫1992" 中外医学社, 350 (1992)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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