• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of transcriptional regulator in lymphocyte

Research Project

Project/Area Number 03670257
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Immunology
Research InstitutionThe Institute of Physical and Chemical Research(RIKEN)

Principal Investigator

MAEKAWA Toshio  RIKEN, Biodesign group, バイオデザイン研究グループ, 研究員 (90201764)

Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1992: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1991: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordstranscriptional regulators / lymphocyte / signal transduction / HIVー1 / エンハンサ-結合蛋白質 / HIVーTF1 / HIVーEP2
Research Abstract

The cAMP response element was originally identified as an inducible enhancer element in the transcriptional regulatory region of the gene which can be transcribed in response to increased cAMP level. On the other hand, CRE can also act as a constitutive enhancer. So far,many CRE-binding proteins have been identified by cDNA cloning. All of them have the DNA-binding domain consisting of a basic amino acid cluster and a leucine zipper(so called B-ZIP structure), and bind to CRE as a homodimer or a heterodimer. Our analyses have indicated that these CRE-binding proteins can be classified into two groups based on their functions. One group involves CREB that was identified by Montiminy's group, and the transactivating capacity of CRE-binding proteins belonging to this group is stimulated by PKA-dependent phosphorylation. The typical member of the second group is CRE-BP1 that was identified by our group, and the transactivating capacity of CRE-BP1 is not stimulated by PKA. The striking feature of CRE-BP1 is that CRE-BP1 forms a heterodimer with c-Jun and that a CRE-BP1/c-Jun heterodimer can bind to CRE. A c-Jun/c-Fos heterodimer(Ap-1)is well known to bind to the TPA response element(TRE). Therefore CRE-BP1 plays an important role for a cross-talk between the cAMP pathway and TPA pathway for intracellular signal transduction.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] NOMURA,N.: "HIV-EP2,a new member of the gene family encoding the human immunodeficiency virus type 1 enhancer binding protein" J.Biol.Chem.266. 8590-8594 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] MATSUDA,S.: "Identification of the functional domains of the transcriptional regulator CRE-EP1" J.Biol.Chem.266. 18188-18193 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Zu,Y.-L.: "Complete putative metal finger and leucine zipper structures of CRE-BP1 are required for the E1A-induced trans-activation" J.Biol.Chem.266. 24134-24139 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] INAGAKI,N.: "c-Jun represses the human insulin promoter activity that depends on multiple cyclic AMP response elements" Proc.Natl.Acad.Sci.USA. 89. 1045-1049 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Zu,Y.-L.: "Transcriptional regulation by a point mutant of adenovirus-2 Ela product lacking DNA binding activity" J.Biol.Chem.267. 20181-20187 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] NOMURA,N.: "Isolation and characterization of a novel member of the gene family encoding the CRE-binding protein CRE-BP1" J.Biol.Chem.268. (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Nomura,N.: "HIV-EP2, a new member of the gene family encoding the human immunodeficiency virus type 1 enhancer binding protein" J.Biol.Chem.266. 8590-8594 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Matsuda,S.: "Identification of the functional domains of the transcriptional regulator CRE-BP1" J.Biol.Chem.266. 18188-18193 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Zu,Y.-L.: "Complete putative metal finger and leucine zipper structures of CRE-BP1 are required for the E1A-induced trans-activation" J.Biol.Chem.266. 24134-24139 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Inagaki,N.: "c-Jun represses the human insulin promoter activity that depends on multiple cyclic AMP response elements" Proc.Natl.Acad.Sci.USA. 89. 1045-1049 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Zu,Y.-L.: "Transcriptional regulation by a point mutant of adenovirus-2Ela product lacking DNA binding activity" J.Biol.Chem.267. 20181-20187 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Nomura,N.: "Isolation and characterization of a novel member of the gene family encoding the CRE-binding protein CRE-BP1" J.Biol.Chem. 268. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Inagaki,N.: "c-Jun represses the human insulin promoter activity that depends on multiple cyclic AMP response elements" Proc.Natl.Acad.Sci.USA. 89. 1045-1049 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Zu,Y.-L.: "Transcriptional regulation by a point mutant of adenovirus-2 Ela product lacking DNA binding activity" J.Biol.Chem.267. 20181-20187 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Nomura,N.: "Isolation and characterization of a novel member of the gene family encoding the CRE-binding protein CRE-BP1" J.Biol.Chem.268. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Someya,Y.: "Two 3'5'-cyclic-adenosine monophospate response elements in the promoter region of the human gastric inhibitory peptide gene." FEBS Lett.317. 67-73 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Toshio Maekawa: "USF-related transcription factor,HIV-TF1,stimulates transcription of human immunodeficiency virus-1" Nucleic Acids Research. 19. 4689-4694 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Nobuo Nomura: "HIV-EP2,a new member of the gene family encoding the human immunodeficiency virus type 1 enhancer-binding protein" J.Biol.Chem.266. 8590-8594 (1991)

    • Related Report
      1991 Annual Research Report

URL: 

Published: 1991-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi