• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Role of Antigenic Milieu on the Generation of Autoreactive Immune Repertoire

Research Project

Project/Area Number 03670258
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Immunology
Research InstitutionNational Institute of Neuroscience, NCNP

Principal Investigator

YAMAMOTO Hiroshi  Dept. Immunol., Natl. Inst. Neurosci, NCNP Director, 神経研究所・免疫研究部, 部長 (50127312)

Co-Investigator(Kenkyū-buntansha) TAKEUCHI Tamotsu  Dept. Immunol., Natl. Inst. Neurosci, NCNP Staff Scientist, 神経センター・神経研究所・免疫研究部, 研究員 (50226990)
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1992: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1991: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsrepertoire / idiotype / T cells / thymus / thymic epithelial cells / cell interaction / レパ-トリ- / ス-パ-抗原 / 胸腺細胞
Research Abstract

(1). MOPC104E myeloma idiotype (M104E CRI) is immunogenic in inducing CRI-specific T cells. In order to investigate the CRI dependent T cell repertoire selection mechanisms, M104E heavy chain gene transfected B cell lines were establish-ed. Several T cell lines reactive to the CRI were also established from BALB/c mice. One of them exhibited significant and specific proliferative response against M104E gene transfected cell line. Fine specificity analysis using CDR2 peptide (on which the CRI locates) is now underway. Myelin associated glycoprotein (MAG) gene transfected B cell lines were also established. The cell lines may sensitize T cells resulted in the induction of T cell mediated autoimmune neuropathy. The T cell repertoire of the effector cells will be investigated.
(2). Thymic epithelial cell line, SL10.3, was established. The cells supports the proliferation of immature thymocytes and of a certain T cell clone. The novel monoclonal antibody (QR6.6) reactive to the T cell clone was raised. QR6.
6 detects 100kd molecule on immature thymocytes. The molecular analysis of the molecule is in progress. On the other hand, monoclonal antibodies reactive to 45kd or 60kd molecule of thymic epithelial cells were also established. A candidate cDNA fragments for the 60kd molecule were cloned. The DNA sequence analysis revealed that the cDNA is coding for a novel molecule. The cloning of full-length cDNA is now underway.
(3). Transfection of various genes coding for self antigens, such as M104E, MAG, myelin basic protein, and stress proteins, into thymic epithelial cells, and immunize them to mice may sensitize or tolerize T cells. This approach will give us a usuful model system to manipulate the self-specific immunity.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Hiroo TAMURA: "The novel monoclonal antibody QR6.6 detects a surface molecule on immature thymocytes and inhibits their proliferation on thymic epithelial cells." Journal of Immunology.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hiroshi YAMAMOTO: "Idiotype-dependent repertoire qeneration in network system." Critical Reviews in Immunology. (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Tamotsu TAKEUCHI: "A 60kd thymic epithelial cell surface protein as a potent molecule mediating the cellular interaction with immature T cells." Cellular Immunology. (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Yasushi MATSUURA: "Establishment of immunoglobulim gene transfected B cell lines." Journal of Cellular Biochemistry. 17B. 212 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Seiichi TAKAHASHI: "Molecular analysis of immunoglobulin heavy chain genes coding for idiotypic and anti-idiotypic antibodies involved in B-B cellular interaction." Micrbiology and Immunology. 36. 855-863 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] TaKetoshi TANIGUCHI: "Inhibition of interferon-γ-dependent induction of major histocompatibility complex II antigen by expressing poly(ADP-ribose)synthetase gene." ADP-Ribosylation Reaction(Springer-Verlag). 141-144 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hiroo TAMURA: "Proliferation of an athymic mouse-derived T-cell clone on thymic stromal cells with interleukin-2." Immunology. 74. 265-270 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hideaki ENZAN: "Immunoelectron microscopic identification of asialo GM1-positive cells in adult rat liver." Virchows Archiv B, Cell Pathology. 60. 389-398 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] H.Tamura, H.Kuzuhara, T.Takeuchi, H.Yamamoto: "The novel monoclonal antibody QR6.6 detects a surface molecule on immature thymocytes and inhibits their proliferation on thymic epithelial cells" J.Immunol.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] H.Yamamoto: "Idiotype-dependent repertoire generation in network system" Crit. Rev. Immunol. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Takeuchi, H.Kuzuhara, H.Yamamoto, et al: "A 60kd thymic epithelial cell surface protein as a potent molecule mediating the cellular interaction with immature T cells" Cell. Immunol. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Y.Matsuura, H,Yamamoto: "Establishment of immunoglobulin gene transfected B cell lines. (abstract)" J. Cell. Biochem. 17B. 212 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] S.Takahashi, Y.Matsuura, H.Yamamoto, et al: "Molecular analysis of immunoglobulin heavy chain genes coding for idiotypic and anti-idiotypic antibodies involved in B-B cellular interaction" Microbiol. Immunol. 36. 855-863 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Taniguchi, T.Tomoda, H.Yamamoto, et al: "Inhibition of interferon-gamma-dependent induction of major histocompatibility complex class II antigen by expressing exogenous poly(ADP-ribose) synthetase gene" ADP-Ribosylation Reactions. 141-144 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] H.Tamura, H.Kuzuhara, H.Yamamoto, et al: "Proliferation of an athymic mouse-derived T-cell clone on thymic stromal cells with interleukin-2" Immunology. 74. 265-270 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] H.Enzan, M.Hiroi, H.Yamamoto, et al: "Immunoelectron microscopic identification of asialo GM1-positive cells in adult rat liver" Virchows archiv B. 60. 389-398 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Y.Numata, Y.Matsuura, H.Yamamoto, et al: "Interleukin-6 positive follicular hyperplasia in the lymph node of a patient with rheumatoid arthritis" Am. J. Hematol. 36. 282-284 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Tomoda, H.Yamamoto, T.Taniguchi, et al: "Fluctuation of gene expression for poly(ADP-ribose) synthetase during hemin-induced erythroid differentiation of human leukemia K562 cells and its reversion process" Biochim. Biophys. Acta. 1088-359-364 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Tomoda, H.Yamamoto, T.Taniguchi, et al: "Enhanced expression of poly(ADP-ribose) synthetase gene in malignant lymphoma" Am. J. Hematol. 37. 223-227 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Taniguchi, S.Takahashi, H.Yamamoto, et al: "Requirement of down-regulation of NAD^+ ADP-ribosyl-transferase for the interferon-gamma-induced activation process of murine macrophage tumor cells" Eur. J. Biochem. 195. 557-562 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hiroshi YAMAMOTO: "Idiotype-dependent repertoire generation in network system." Critical Reviews in Immunology. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Tamotsu Takeuchi: "A 60kd thymic epithelial cell surface protein as a potent molecule mediationg the cellular interaction with immature T cells." Cellular Immunology. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Yasushi Matsuura: "Establishment of immunoglobulin gene transfected B cell lines." Journal od Cellular Biochemistry. 17B. 212- (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Seiichi Takahashi: "Molecular analysis of immunoglobulin heavy chain genes coding for idiotypic and anti-idiotypic antibodies involved in B-B cellular interaction." Microbilogy and Immunology. 36. 855-863 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Taketoshi Taniguchi: "Inhibition of interferon-γ-dependent induction of major histocompatibility complex class 2 antigen by expressing poly(ADP-ribose) synthetase gene." ADP-Ribosylation Reaction (Springer-Verlag). 141-144 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Yukiko NUMATA: "Interleukin-6 positive follicular hyperplasia in the lymph node of a patient with rheumatoid arthritis." American Journal of Hematology. 36. 282-284 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hideaki ENZAN: "Immunoelectron microscopic identification of asialo GMl-positive cells in adult rat liver." Virchows Archiv B,Cell Pathology. 60. 389-398 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Takashi TOMODA: "Enhanced expression of poly(ADP-ribose)synthetase gene in malignant lymphoma." American Journal of Hematology. 37. 223-227 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hiroo TAMURA: "Proliferation of an athymic mouse-derived T-cell clone on thymic stromal cells with interleukin-2." Immunology. 74. 265-270 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Seiichi TAKAHASHI: "Molecular analysis of immunoglobulin heavy chain genes coding for idiotypic and anti-idiotypic antibodies involved in B-B cellular interaction." Microbiology and Immunology. (1992)

    • Related Report
      1991 Annual Research Report

URL: 

Published: 1991-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi