| Project/Area Number |
03670276
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Hygiene
|
|---|
| Research Institution | Kawasaki Med. School |
|---|
Principal Investigator |
UEKI Ayako Kawasaki Med. School, Hygiene, Professor, 医学部, 教授 (10069031)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
UEKI Ayako Kawasaki Med. School, Hygiene, Professor (10069031)
|
|---|
| Project Period (FY) |
1991 – 1993
|
| Project Status |
Completed (Fiscal Year 1993)
|
| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1993: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1992: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1991: ¥700,000 (Direct Cost: ¥700,000)
|
|---|
| Keywords | endothelial cells / complement / cytokines / 補体成分産生 |
|---|
| Research Abstract |
Human umbilical cord vein endothelial cells (HUVEC) are known to synthesize and secrete complement components, and we reported previously the synthesis of Complement component 3 (C3). When we assayd the amount of C3 and factor B (Bf) with ELISA in culture supernatant of HUVEC stimulated with several king of cytokines, TNF-alpha had the strongest effect on the synthesis of complement components, and TNF-beta, IL-1beta and IFN-gamma also demonstrated the stimulating effect. There results were in accoydance with the mRNA level in the cells examined with Nothern blotting method. Combination of TNF-alpha and IL-1beta or TNF-alpha and IFN-gamma had synergistic effects on the release of C3 or Bf respectivly.
|
