| Project/Area Number |
03670294
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Legal medicine
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| Research Institution | GUNMA UNIVERSITY |
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Principal Investigator |
MATSUKI Takasumi GUNMA UNIV.SCHOOL MED.ASSOC.PROF., 医学部, 助教授 (10126617)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAJIMA Tamako GUNMA UNIV.SCHOOL MED.ASSISTANT, 医学部, 助手 (40008561)
FURUKAWA Ken GUNMA UNIV.SCHOOL MED.PROFESSOR, 医学部, 教授 (90008227)
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| Project Period (FY) |
1991 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1992: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1991: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | BLOOD GROUP / GENOTYPE / PHENOTYPE / ABO |
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| Research Abstract |
(1) DEVELOPMENT OF PHENOTYPE DETERMINATION METHOD FROM DNA
WE DEVELOPED A NEW SIMPLE AND RELIABLE METHOD FOR THE DETERMINATION OF O-GENE FROM DNA SAMPLES.DNA WAS EXTRACTED BY SDS-PROTEINASE K-PHENOL METHOD OR BY CHELEX-100 METHOD.O-GENE WAS AMPLIFIED BY ALLELE SPECIFIC POLYMERASE CHAIN REACTION.THE AMPLIFIED DNA WAS DETECTED BY 15% PAGE AND SILVER NITRATE STAINING.DNA FROM UNRELATED TWENTY-FIVE O PHENOTYPE PERSONS HAD O-GENE PRODUCT, AND THOSE FROM TEN AB PHENOTYPE PERSONS HAD NO PRODUCT.
(2) SEROLOGIC STUDY OF ABO PHENOTYPE AND FAMILY ANALYSIS
SIXTY-FIVE SAMPLES FROM NINETEEN FAMILIES AND UNRELATED THREE HUNDRED EIGHTY-FIVE SAMPLES WERE COLLECTED IN THESE THREE YEARS.THERE WAS NO AA OR BB GENOTYPE PERSON FROM OUR SEROLOGIC AND FAMILY STUDIES.
(3) FACTOR(S) AFFECTING ANTIGEN EXPRESSION FROM GENOME SEQUENCE IN THE ABO SYSTEM
WE HAVE NO RESULT ON THIS SUBJECT IN THIS PERIOD.WE NEED MORE STUDIES TO GET THE ANSWER.
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