ANALYSIS OF B-CELL DEFECTS IN COMMON VARIABLE IMMUNODEFICIENCY AT GENE LEVEL.
Project/Area Number |
03670324
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Research Category |
Grant-in-Aid for General Scientific Research (C)
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Allocation Type | Single-year Grants |
Research Field |
内科学一般
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Research Institution | Ehime University |
Principal Investigator |
SAIKI Osamu Ehime Univ. Medicine Microbiology, Associate Prof., 医学部, 助教授 (40162177)
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Project Period (FY) |
1991 – 1992
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Project Status |
Completed (Fiscal Year 1992)
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Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1992: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1991: ¥1,500,000 (Direct Cost: ¥1,500,000)
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Keywords | Immunodeficiency / B-cell differentiation / Staphylococcus aureus / antibody formation |
Research Abstract |
WE SUMMARIZE HERE WITH TWO FINDINGS OBTAINED DURING THE TWO-YEAR-RESEARCH PROJECT "ANALYSIS OF B-CELL DEFECTS IN COMMON VARIABLE IMMUNODEFICIENCY AT GENE LEVEL". 1. INDUCTION OF IG SECRETION IN HUMAN B CELL BY PROTEIN A-DEFICIENT STAPHYLOCOCCUS AUREUS WOOD 46 STRAIN (SAW) WAS EXAMINED. SAW INDUCED AS MUCH IG SECRETION AS PROTEIN A-RICH SA COWAN-I (SAC) WHEN IL-2 WAS PRESENT IN THE CULTURE. MECHANISM OF IG INDUCTION BY SAC AND SAW WAS COMPARED. SAW DID NOT INDUCED B CELL PROLIFERATION NOR TYROSINE PHOSPHORYLATION. HOWEVER, SAC INDUCED BOTH B CELL PROLIFERATION AND TYROSINE PHOSPHORYLATION. IN FURTHER EXPERIMENTS, INDUCTION OF IL-2R AND IgMmRNA EXPRESSIONS WERE EXAMINED. SAW BY ITSELF INDUCED IL-2R AND IgMmRNA EXPRESSIONS WITHOUT AFFECTING EXPRESSION OF MEMBRANE-TYPE IgMmRNA. THESE RESULTS SUGGEST SHOW THAT SAW ACTIVATES HUMAN B CELLS IN A QUITE DIFFERENT MANNER FROM SAC BY UP-REGULATING THE EXPRESSION OF SECRETORY TYPE IgMmRNA WITHOUT AFFECTING CELL PROLIFERATION NOR TYROSINE PHOSPHORYLATION, PROPOSING A DISTINCT B CELL ACTIVATION MODEL FROM SAC. 2. WE ESTABLISHED ABOUT 30 B CELL LINE FROM PATIENTS OF B CELL FUNCTIONAL DEFECTS INCLUDING BROOM SYNDROME AND IMMUNODEFICIENCY WITH HYPER IgM. IN PATIENTS WITH HYPER IgM, X-CHROMOSOME INACTIVATION ASSAY WAS CARRIED OUT, AND MOTHER WAS FOUND TO BE A CARRIER, FOLLOWINGLY DIAGNOSIS X-LINKED IMMUNODEFICIENCY WITH HYPER IgM WAS FOUND.
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Report
(3 results)
Research Products
(13 results)