| Project/Area Number |
03670328
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
内科学一般
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| Research Institution | Kyusyu University |
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Principal Investigator |
NOBUNAGA Masashi Medical Institute of Bioregulation, Kyusyu University, Professor, 生体防御医学研究所, 教授 (40038899)
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| Co-Investigator(Kenkyū-buntansha) |
HASHIMOTO Michi Medical Institute of Bioregulation Kyusyu University, Lecturer, 生体防御医学研究所, 助手 (00237950)
WADA Tetsuya Medical Institute of Bioregulation, Kyusyu University, Lecturer, 生体防御医学研究所, 助手 (30220958)
EZAKI Ichiko Medical Institute of Bioregulation, Kyusyu University, Lecturer, 生体防御医学研究所, 助手 (40037453)
NONAKA Shirou Medical Institute of Bioregulation, Kyusyu University, Lecturer, 生体防御医学研究所, 助手 (60198603)
SHINGU Masao Medical Institute of Bioregulation, Kyusyu University, Associate Professor, 生体防御医学研究所, 助教授 (10038523)
塩川 左斗志 九州大学, 生体防御医学研究所, 助手 (20215940)
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| Project Period (FY) |
1991 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1992: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1991: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | osteoclasts / rheumatoid arthritis / interleukin 1B / interleukin 4 / interleukin 6 / TIMP-1 / インターロイキン1beta / 骨破壊 / TIMP / 骨吸収 / ICAM-1 / VCAM-1 / IL-1β / サイトカイン / インタ-ロイキン1 |
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| Research Abstract |
The amounts of stromelysin (MMP-3) and TIMP-1 production by osteoclasts obtained from the bone marrow of rheumatoid arthritis(RA) patients were higher than those from osteoarthritis(OA) patients, but the difference was not significant. IL-1B significantly enhanced MMP-3 production in osteoclasts, but the addition of IL-4 abolished the enhancing effect of IL-1B.IL-E also significantly suppresed MMP-3 production by osteoclasts. IL-B increased TIMP-1 production by osteoclasts, and IL-4 markedly enhanced TIMP-1 production by osteoclasts. IL-6 showed similar effect.
Osteoclasts released calcium from pre-labelled osteoblasts. When the calcium releasing activity of osteoclasts were compared between RA and OA, osteoclasts form RA patients released higher amounts of calcium but the difference was not significant.
These results suggest that not only bone destruciton factor but also bone protective factor are modulated in RA, and that these functional changes of osteoclasts in RA are related to cytokined such as IL-1, IL-4 and IL-6.
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