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The analysis of immune response and cytotoxic mechanism with use of anti-ICAM-1 monoclonal antibodies

Research Project

Project/Area Number 03670330
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 内科学一般
Research InstitutionSapporo Medical College

Principal Investigator

TSUJISAKI Masayuki  Sapporo Med. College, Dept. of Int. Med., Instructor, 医学部, 助手 (50217311)

Co-Investigator(Kenkyū-buntansha) YACHI Akira  Sapporo Med. College, Dept. of Int. Med., Prof., 医学部, 教授 (50045324)
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1992: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1991: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsadhesion modecule / ICAM-1 / circulating antigen / cytotoxic mechanism / metastasis and invasion of cancer cells / ICAMー1 / モノクロ-ナル抗体
Research Abstract

A new monoclonal antibody (MoAb) HA58 (IgGl) was prepared, which recognizes the binding site on the intercellular adhesion molecule-1 (ICAM-1) antigen to the lymphocyte function-associated antigen-1 (LFA-1). The double-determinant immunoassay (DDIA) was established with use of MoAb HA58 and another anti-ICAM-1, MoAb CL207, to detect the soluble, shedding ICAM-1 antigen. The incidence of positivity for ICAM-1 antigen in malignant diseases was higher than that in benign diseases or in healthy controls. Immunostaining of cancerous tissue with MoAb HA58 showed that the ICAM-1 antigen was expressed not only on malignant cells but also on stromal cells (mainly fibroblasts) near cancer nests, and the stromal cells immediately adjacent to cancer nests, in comparison to those cells farther away from the nests, have a higher ICAM-1 expression. We speculate that tumor cells activate T cells to produce cytokines (e.g.IFN-gamma), resulting in strong expression of the ICAM-1 on surrounding fibroblasts, as well as on tumor cells. Shedding ICAM-1 antigen may also block the attachment of cytotoxic T cells and/or NK cells to cancer cells, since LFA-1 could be blocked with soluble ICAM-1. This could be one of the escape mechanisms of cancer cells from the immunosurveillance system of the host.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] K.Imai: "Increased circulating intercellular adhesion molecule-1 in adult T cell leukemia patients" Int.J.Hematology. 56. 239-240 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] N.Shijubo: "Circulating ICAM-1 antigeu in seso of patients urth idiopathic pulmonery fibrosis" Clin.exp.Immunol.89. 58-62 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 谷内 昭: "CD54/ICAM-1分子" Medical Immunology. 24. 573-580 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 辻崎 正幸: "キラーリンパ球の動態" 臨床免疫. 25. 175-182 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 辻崎 正幸: "腫瘍(細胞接着因子の面から)" 日本臨床免疫学会誌. 15. 586-592 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 辻崎 正幸: "悪性腫瘍における接着分子" BIOmedica. 7. 37-41 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Tsujisaki,M., Imai,K., Hirata,H., Hanzawa,Y., Masuya,J., Nakano,T., Sugiyama,T., Matsui,M., Hinoda,Y. and Yachi,A.: "Detection of circulating intercellular adhesion molecule-1 antigen in maligant diseases." Clin. Exp. Immunol.85. 3-8 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shijubo,N., Imai,K., Aoki,S., Hirasawa,M., Sugawara,H., Koba,H., Tsujisaki,M., Sugiyama,T., Hinoda,Y., Yachi,A., Asakawa,M. and Suzuki,A.: "Circulating intercellular adhesion molecule-1 (ICAM-1) antigen in sera of patients with idiopathic pulmonary fibrosis." Clin. Exp. Immunol.89. 58-62 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Imai,K., Nakano,T., Motoya,S., Araki,K., Tsujisaki,M., Hinoda,Y. and Yachi,A.: "Increased circulating ICAM-1 in adult T cell leukemia patients." Int. J. Hematology. 56. 239-240 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] K.Imai: "Increased circulating inter cellular adhesion molecule-1 in adult T cell leukemia patients" Int.J.Hematology. 56. 239-240 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] N.Shijulo: "Circulating ICAM-1 antigen in sera of patients with idiopathic pulmoney filrosis" Clin.exp.Immunol.89. 58-62 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 谷内 昭: "CD54/ICAM-1分子" Medical Immunology. 24. 573-580 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 辻崎 正幸: "キラーリンパ球の動態" 臨床免疫. 25. 175-182 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 辻崎 正幸: "腫瘍(細胞接着因子の面から)" 日本臨床免疫学会誌. 15. 586-592 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 辻崎 正幸: "悪性腫瘍における接着分子" BIO medica. 7. 37-41 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Tsujisaki,M.et al.: "The analysis of internal imageーbearing antiーidiotypic monoclonal antibody in relation to carcinoembryonic antigen" Journal Immunology. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Tsujisaki,M.et al.: "Detection of circulating intercellular adhesion moleculeー1 antigen in malignant diseases." Clinical Experimental Immunology. 85. 3-8 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Tsujisaki,M.et al.: "Induction of antigen specific immune response with use of antiーidiotypic monoclonal antibodies to antiーCEA antibodies" Cancee Research. 51. 2599-2604 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Sugiyama,T.et al.: "Conformational structure of a monoclonal antiーidiotypic antibody to the monoclonal antiーadenocarcinoma associated carbohydrate antibody YH206" Journal Immunology. 146. 3097-3101 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Tsujisaki,M.et al.: "New assay to detect shedding antigen with use of antiーidiotypic monoclonal antibodies." J.of Climical Laboratory Analysis. 5. 14-20 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Tsujisaki,M.et al: "Preparation and Characterization of monoclonal antibodies specific to synthetic peptide of carcinoembryonic antigen." International J.of Cancer. 47. 267-273 (1991)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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