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The role of VLA proteins and fibronectin in the activation of synovial cells from rheumatoid arthritis.

Research Project

Project/Area Number 03670333
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 内科学一般
Research InstitutionKagoshima University (1992-1993)
Saitama Medical University (1991)

Principal Investigator

MATSUYAMA Takami  School of Medical Zoology, Professor, 医学部, 教授 (30145479)

Co-Investigator(Kenkyū-buntansha) 小出 純  埼玉医科大学, 医学部, 講師 (70178193)
竹内 勤  埼玉医科大学, 医学部, 講師 (50179610)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1992: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1991: ¥800,000 (Direct Cost: ¥800,000)
KeywordsRheumatoid Arthritis / VLA / Fibronectin / Synovium / VCAM-1 / フイブロネクチン / 滑膜細胞フィブロネクチン / 形態変化 / チロシンリン酸化 / 活性化
Research Abstract

Title : The role of VLA proteins and fibronectin in the activation of synovial cells from rheumatoid arthritis.
In this study, our aim was to clarify whether the interaction of the very late antigen (VLA) on synovial cells from rheumatoid arthritis patients and that ligand, fibronectin is involvd in the pathogenesis of rheumatoid arthritis synovitis. First, we examined the expression of VLA proteins (VLA2, 3, 4, 5) on synovial tissues. VLA3, 4 and 5 proteins were expressed on the synovial lining and sublining cells. Lymphocytes mainly expressed VLA-4. Endothelial cells expressed VLA2, 3 and 5 proteins.
When we compared the expression of VLA-4 protein among peripheral blood T cells, synovial T cells and synovial fluid T cells, we found the increased expression of VLA-4 on synovial fluid T cells, suggesting the existence of VLA-4 inducing substance in synovial fluid. Next, we examined whether fibronectin and that fragments affect the proliferation of synovial cells. Although they did not so, they induced the shape change of synovial A cells. When we detected the phosphorylated proteins in synovial cells after the stimulation of fibronectin, we did not get the constant pattern. Recently it was reported that the vascular cell adhesion molecule-1 (VCAM-1) was the other ligand of VLA-4 protein. Thereby, we examined the expression of VCAM-1 in the synovial tissues from rheumatoid arthritis patients. That expression was shown on the synovial lining and sublining cells. Since most of these cells express the VLA-4 protein, the interaction of these cells might be attributed to the binding of VLA-4 and VCAM-1 molecules.

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] 松山隆美: "VLA" 臨床検査. 37. 308-309 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 松山隆美: "接着分子と炎症" カレントテラピー. 11. 37-40 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Matsuyama et al: "Cytochalasins enhance the proliferation of CD4 cells through the CD3-Ti antigen receptor complex or CD2 molecule by affecting early events of cell activation." J.Immunology.146. 3736-3741 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Matsuyama et al: "CD45 isoform associated with distinct functions of from CD4 cells derived unusual healthy donors lacking CD45RA-T lymphocytes." Cellular Immunology.137. 406-419 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Matsuyama et al: "Abnormality of signal transduction in synovial T cells from rheumatoid arthritis." Igakunoayumi.161. 562-566 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Matsuyama et al: "alpha_1 acid glycoprotein expression in human leukocytes : possible correlation between alpha_1-acid glycoprotein and inflammatory cytokines in rheumatoid arthritis." Inflammation.17(1). 33-45 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Matsuyama: "Cytochalasins enhance the proliferation of CD4 cells shrough the CD3-Ti antigen receptor complex or CD2 molecule by affecting early events of cell activation" J.Immunology.146. 3736-3741 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] T.Matsuyama: "CD45 isoform associated with distinct functions of CD4 cells derived from unusual healthy donors lacking CD45RA-T lymphocytes." Cellular Immunology.137. 406-419 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] T.Nakamura: "alpha_1 acid glycoprotein expression in human leukocytes : possible correlation between alpha_1-acid glycoprotein and inflammatory cytokines in rheumatoid arthritis." Inflammation.17. 33-45 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 松山隆美: "beta細胞の胚中心への接着にはVLA-4とINCAM-110が関与している" 臨床免疫. 24. 133-136 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 松山隆美: "RAにおけるT細胞とシグナル伝達異常" 医学のあゆみ. 161. 562-566 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 松山隆美: "細胞外マトリックスと免疫系の相互作用" 臨床免疫. 25. 85-90 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 松山 隆美: "B細胞の胚中心への接着にはVLA-4とINCAM-110が関与している" 臨床免疫. 24. 133-136 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 松山 隆美: "細胞外マトリックスと免疫系の相互作用" 臨床免疫. 25. 85-90 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 松山 隆美: "RA滑膜病変におけるDR抗原の役割" 平成3年度厚生省リウマチ調査研究事業研究報告. 99-104 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 伊藤 功・天野 宏一・小出 純・竹内 勤・松山 隆美・安倍 達: "臨床的寛解をみた慢性関節リウマチに関する研究" リウマチ. 32. 47-51 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 松山 隆美: "RAにおけるT細胞とシグナル伝達異常" 医学のあゆみ. 161. 562-566 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Nakamura T, Board PG, Matsuyama T, Matsuda T.: "α_1-ACID GLYCOPROTEIN EXPRESSION IN HUMAN LEUKOCYTES : Possible Correlation between α_1-Asid Glycoprotein and Inflammatory Cytokinesin Rheumatoid Arthritis" Inflammation.

    • Related Report
      1992 Annual Research Report
  • [Publications] 松山 隆美: "慢性関節リウマチ滑膜病変とVLA抗原" 医学のあゆみ. 156. 453-456 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 松山 隆美: "リウマチと接着分子異常" Medical Immunology. 20. 601-605 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 松山 隆美: "炎症と細胞表面接着分子" 医学のあゆみ. 159. 363-365 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 松山 隆美: "VLA検査" 臨床検査. 35. 1277-1281 (1991)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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