| Project/Area Number |
03670334
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
内科学一般
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| Research Institution | Saitama Medical Center, Saitama Medical School |
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Principal Investigator |
KOIDE Jun Saitama Medical Center, Saitama Medical School 2nd Department of Medicine., 医学部, 講師 (70178193)
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| Project Period (FY) |
1991 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1993: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1992: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1991: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | Behcet's disease / Strepto.sanguis / KTH-1-Ag / gamma delta -TCR^+Tcell / TNF alpha / Cytotoxicity / Ca^<++>-influx / gammadelta-TCR+T細胞 / TNFalpha / Ca〓濃度 / γδ細胞クローン / CD4^+T細胞ライン / NK細胞ライン / 細胞障害能 / 好中球活性酸素産生能 / CD44分子 / ベ-チェット病 / γδ細胞 / T細胞クロ-ン / KTHー1細菌抗原 / 抗原特異的増殖反応 / DQ抗体 |
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| Research Abstract |
IN the present study, we established also KTH-1-reactive three gamma delta +T cell clones and two CD4+ T cell clones more easily derived from peripheral blood in five patients with Behcet's disease. We show that these gamma delta clones express much more molecules of CD44 and VLA-1 than that of those CD4 clones, all of clones have proliferated vigorously in the response to KTH-1, but not rHSP(65KD), and the capacity of cytokine-production indicated the highest amounts of TNF alpha secreted by KTH-1-stimulated gamma delta clones, in contrast to moderate amounts of IL2 secreted by KTH-1-stimulated CD4 clones. Of interest was that the significant killing activity was shown by only gamma delta clones against NK-sensitive target cells, autologous and allogeneic EBV-transformed B cell lines, and this antivity was enhanced by a part of antibodies to CD44, or inhibited by tyrosine kinase-inhibitor(Herbimycin)in a different fashion. The most striking evidence was that there was quite difference of the changes in [Ca2+]i after KTH-1-stimulation of between gamma delta clones and CD4 clones by examination of single cell image analysis on ACAS.We suspected that these results indicated gamma delta +T cells, but not CD4+ T cell, have a central pathogenic role on the development of chronic inflammation after KTH-1-infection in Behcet's disease.
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