| Project/Area Number |
03670341
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
内科学一般
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| Research Institution | NIHON UNIVERSITY |
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Principal Investigator |
SAWADA Shigemasa NIHON UNIV.ASSOCIATE PROF.SCH.OF MED., 医学部, 助教授 (40130520)
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| Co-Investigator(Kenkyū-buntansha) |
MITAMURA Ko NIHON UNIV.ASSOCIATE SCH.OF MED., 医学部, 助手
TAKEI Masami NIHON UNIV.ASSOCIATE SCH.OF MED., 医学部, 助手
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| Project Period (FY) |
1991 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1992: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1991: ¥600,000 (Direct Cost: ¥600,000)
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| Keywords | rheumatoid arthritis / EB virus / sCD23 / sinovial cell / in situ hybridization / polymerase chain reaction / osteoarthritis / mRNA / 変形性関節症 / EBウィルス / RT-PCR法 / RA滑膜 / EBNA-1 mRNA / プライマー / EpsteinーBarrウイルス / EBウイルスDNA / EBウイルスRNA |
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| Research Abstract |
This project demonstrated that EB virus(EBV)-DNA was detected by polymerase chain reaction(PCR) method using EBNA-1 primers in sinovial tissue from the patients with rheumatoid arthritis(RA), but not detected mRNA by reverse transcriptase PCR(RT-PCR), and the concentration of solble CD23(sCD23) which was trans-activated by EBNA-2 gene, was significant higher in sera from patients with RA than normal individuals. Furthermore we found internal repeat-1 EBV-DNA signals by in situ hybridization in synovial cells which might be spindle type, from type III synovium of a patient with RA but not in another 3 RA and a osteoarthritis patients.
This is a first report that has demonstrated the existence of EBV in sinovial cells from patient with RA.Further investigation will clarify the association between EBV and pathgenesis of RA.
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