| Project/Area Number |
03670342
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Gastroenterology
|
|---|
| Research Institution | Asahikawa Medica College |
|---|
Principal Investigator |
SEKIYA Chihiro Asahikawa Med.Col., Internal Medicine III, Lecturer, 医学部, 講師 (30109384)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TANIGUCHI Naoyuki Osaska University Med. School, Biochemistry, Professor, 医学部, 教授 (90002188)
ONO Minoru Asahikawa Med.Col., Internal Medicine III, Assistant Professor, 医学部, 助手 (60185650)
|
|---|
| Project Period (FY) |
1991 – 1993
|
| Project Status |
Completed (Fiscal Year 1993)
|
| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1992: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1991: ¥1,200,000 (Direct Cost: ¥1,200,000)
|
|---|
| Keywords | primary biliary cirrhosis / free radicals / manganese-superoxide dismutase / Mn‐SOD / Mnーsuperoxide dismutase / 活性酸素 / フリ-ラジカル / サイトカイン |
|---|
| Research Abstract |
Serum manganese-superoxide dismutase (Mn-SOD) was determined in patients with various liver diseases with an enzyme-linked immunosorbent assay using a specific monoclonal antibody.
The serum Mn-SOD level in patients with primary biliary cirrhosis (PBC) was significantly increased compared with those of other liver diseases, including chronic viral hepatitis, liver cirrhosis, autoimmune hepatitis, obstructive jaundice, fatty liver and idiopathic portal hypertension. Administration of ursodeoxycholic acid caused the significant decrease of serum Mn-SOD level in PBC patients. Immunostaining of a liver biopsy specimen from the patients with PBC revealed increased expression of the enzyme protein in damaged hepatocytes and degenerated epithelial cells of interlobular bile ducts.
|
