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Molecular mechanisms of Tumor-cytolysis by CTL against gastric carcinoma.

Research Project

Project/Area Number 03670348
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Gastroenterology
Research InstitutionUniversity of Tsukuba.

Principal Investigator

KOYAMA Shohei  Dept. Int. Med. Assistant Prof. Univ. of Tsukuba, 臨床医学系, 講師 (00110502)

Co-Investigator(Kenkyū-buntansha) FUKAO Katashi  Dept. Surg. Prof. Univ. of Tsukuba., 臨床医学系, 教授 (50091921)
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1992: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1991: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsgastric carcinoma / killer T cells (CTL) / suppressor cells / ICAM-1 / Adhesion molecules / LAK cells / TIL / キラ-T細胞 / サプレッサ-T細胞 / ICAMー1 / LFAー1
Research Abstract

1) Malignant pleural or peritoneal effusion-associated lymphoid (EAL) cells from patients with advanced carcinoma were cultured with autologous carcinoma with autologous carcinoma cells in the presence of rIL-2 or T-cell growth factor (TCGF: culture supernatant of PHA-stimulated spleen cells). Consequently, the majority of killer-effector cells against allogeneic cells in rIL-2-activated EAL cells from cancer patients showed CD4^+Leu8^-phenotype. In contrast, the cytolytic activity against autologous or allogeneic tumor cells in TCGF-activated EAL cells was mediated by CD8^+CD11b^- and CD8^+CD28^+ effector cells.
2) The CTL activity was mediated by CD2, CD3/TCR complex, CD8 and ICAM-1 molecules. However, LAK activity was mediated by CD2, CD4 and ICAM-1, but not by CD3/TCR, and HLA-Class 1 antigen.
3) TCGG-activated suppressor (LAS) effector cell populations responsible for suppression of cell-mediated antitumor immunity reside within CD8^+CD11b^- T cells.
4) Circulating suppressor cells with surface phenotype of CD8^+ or possible CD8^-CD11b^+ from freshly prepared PBL in patients with advanced gastric carcinoma are noted. The suppression of immunity was clearly directed at the effector process performed by CTL or LAK cells.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] 海老原 次男,酒井 紀恵,深尾 立,小山 捷平: "新しいモノクローム抗体、抗S6F1抗体を用いた進行癌患者の免疫応答の解析" Oncologia. 24(2). 102-107 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 海老原 次男,酒井 紀恵,小山 捷平: "サイトカインを用いて癌性胸・腹水リンパ球より誘導されるKiller細胞の細胞障害性とそのeffector細胞解析" Biotherapy. 5(6). 1158-1162 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] EBIHARA T.,SAKAI N.,KOYAMA S.: "Suppression by sorted CD8^+DD11b^- cells from T-cell growth factor-activated peripheral blood lymphocytes or cytolytic activity against tumour in patients with gastric carcinoma." European J.Cancerarcinoma.27. 1654-1657 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shohei KOYAMA et al.: "Early extramedullary plasmacytoma confined to the lamina propria of the gastric mucosa." Jpn J. Clin.Oncology. 22. 136-141 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shohei KOYAMA et al.: "Expression of inntercellular adhesion molecule l(ICAM-l)during thedevelopment of invasion and/ormetastasis of gastric carcinoma." J.Cancer Re.Clin.Oncology. 118. 609-614 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shohei KOYAMA et al.: "Immunochemotherapy" Int.medicine. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Ebihara T., Sakai N., Fukao K., Koyama S.: "An analysis of immune response in patients with advanced carcinoma by mean of a new monoclonal antibody, ant-S6F1 antibody." Oncologia. 24(2). 102-107 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Ebihara T, Sakai N., Koyama S.: "Killer cells induced from carcinomatous pleural and peritoneal lymphoid cells using cytokines: Their cytotoxic activity and an analysis of the effector cells." Biotherapy. 5(6). 1158-1162 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Ebihara T., Sakai N., Koyama S.: "Suppression by sorted CD8^+CD11b^- cells from T-cells from T-cell growth factor-activated peripheral blood lymphocytes on cytolytic activity against tumor in patients with gastric carcinoma." Eur J Cancer. 27(12). 1654-1657 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Koyama S. et al: "Early extramedullary plasmacytoma confined to the lamina propria of the gastric mucosa." Jpn J. Clin. Oncology. 22(2). 136-141 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Koyama S. et al.: "Expression of intercellular adhesion molecule 1 (ICAM-1) during the development of invasion and/or metastasis of gastric carcinoma." J. Cancer Res, Clin. Oncology. 118. 609-614 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Koyama S.: "Immunochemotherapy" Int. Medicine. (12). (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 海老原 次男,酒井 紀恵,深尾 立,小山 捷平: "新しいモノクローナル抗体,抗S6F1抗体を用いた進行癌患者の免疫応答の解析" Oncologia. 24(2). 102-107 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] 海老原 次男,酒井 紀恵,小山 捷平: "サイトカインを用いて癌性胸腹水リンパ球より誘導されるkiller細胞の細胞障害活性とそのeffector細胞の解析" Biotherapy. 5(6). 1158-1162 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Ebihara,T.,Sakai,N. Koyama,S.: "Suppression by sorted CD8^+CDllb^- cells from T-cell growth factor-activated peripheral blood lymphocytes on cytolytic activity against tumour in patients with gastric carcinoma." European J.Cancer. 27. 1654-1657 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Shohei Koyama et al: "Early extramedullary plasmacytoma confined to the lamina propria of the gastric mucosa." Jpn J.Clin.Oncology. 22. 136-141 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Shohei Koyama et al.: "Expression of intercellular adhesion molecule 1 (ICAM-1)during the development of invasion and/or metastasis of gastric carcinoma." J.Cancer Res.Glin.Oncology. 118. 609-614 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Shohei Koyama et al.: "Immunochemotherapy" Int.Medicine. 31. (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 海老原 次男,酒井 紀恵,深尾 立,小山 捷平: "新しいモノクロ-ナル抗体,抗S6F1抗体を用いた進行癌患者の免疫応答の解析" Oncologia. 24(2). 102-107 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 海老原 次男,酒井 紀恵,小山 捷平: "サイトカインを用いて癌性胸・腹水リンパ球より誘導されるKiller細胞の細胞障害活性とそのeffector細胞の解析" Biotherapy. 5(6). 1158-1162 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Ebihara,N.Sakai,S.Koyama.: "Suppression by sorted CD8^+CD11b^- cells from Tーcell growth factorーactivated peripheral blood lymphocytes on cytolytic activity against tumour in patients with gastric carcinoma." European J.Cancer. 27(12). 1654-1657 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] S.Koyama,T.Ebihara,K.Fukao: "Expression of intercellular adhesion molecule(ICAMー1)during the development of invasion and/or metastasis of gastric carcinoma."

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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