| Project/Area Number |
03670350
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Chiba University |
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Principal Investigator |
IMAZEKI Fumio Chiba University, Assistant, 医学部附属病院, 助手 (40223325)
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| Co-Investigator(Kenkyū-buntansha) |
NOSE Haruhiko Chiba University Resident, 医学部, 医員
TADA Minoru Chiba University Resident, 医学部, 医員
小俣 政男 千葉大学, 医学部, 講師 (90125914)
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1992: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1991: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | p53 gene / Anti-oncogene / Hepatocellular carcinoma / Polymerase chain reaction / Direct nucleotide sequencing / Loss of heterozygosity / Southern blot hybridization / p53 / polymerase chain reaction(PCR) / restriction fragment length polymorphism(RFLP) |
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| Research Abstract |
We analyzed 20 hepatocellular carcinomas (HCC) form Japan for alteration of the p53 gene by restriction fragment length polymorphisms and for nucleic acid mutations by polymerase chain reaction with direct sequencing. Alterations associated with the p53 gene were found in 6 out of 20 HCCs (30%). Allelic loss of chromosome 17p occurred in 5 out of 14 informative (heterozygous) cases (36%). Mutations in the p53 gene were detected in 3 cases (15%), at codons 176 (exon 5), 236 (exon 7) and 294 (exon 8), which were different from the HCC cases from China and South Africa, where point mutations in the p53 gene were reported at the same codon 249 in half of the cases, and where aflatoxin food contamination and hepatitis B virus infection are recognized risk factors of HCC. No p53 gene alterations were found in smaller HCCs (<3 cm) or at earlier stages. In Japan, p53 gene alterations seem to be a late event in the progression of hepatocarcinogenesis, which is often associated with persistent infection by the hepatitis C or B virus, but not usually with exposure to aflatoxin.
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