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Mechanism of bile duct injury in autoimmune cholangitis in senescent mice

Research Project

Project/Area Number 03670364
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Gastroenterology
Research InstitutionEhime University

Principal Investigator

ONJI Morikazu  Ehime Univ. University Hospital, Assistant Professor., 医学部附属病院, 講師 (10112260)

Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1992: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1991: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordssenescent mice / CD8 positive cell / primary biliary cirrhosis / 樹状細胞 / 原発性胆汁性肝硬変モデル・動物 / 胆管障害機構
Research Abstract

The biliary lesions that developed spontaneously in senescent female C57BL/6Nrj mice were investigated. Degeneration of the bile duct epithelium was observed in 12 of 13 mice (92%), destruction of bile duct epithelial cells was seen in 6 of 13 mice (46%) and chronic non-suppurative destructive chonangitis was found in 3 of 13 mice (23%). The biliary lesions were characterized by prominent round cell infiltrates in the portal areas. Extra-hepatic lesions such as sialoadenitits were observed in 6 (46%)and pancreatitis in 7 mice (53%). These lesions were not observed in male C57BL/6, young female C57BL/6, or ICR mice. By transferring the splenic cells of senescent female C57BL/6 mice to 6 week old females, the biliary lesions could be transferred at the rate of 6/9 mice. N418 positive dendritic cells infiltrated into portal areas and their processes contacted with lymphocytes. The lymphocytes infiltrating in the bile ducts were CD8 positive. Moreover, ultrastructural immunocytochemical analysis of lymphocytes infiltrating bile duct epithelia revealed that CD8 positive lymphocytes often formed broad contacts with the epithelial cells. The epithelial cells isolated from gallbladder of young C57BL/6 mice, were cultured with splenic cells of senescent female C57BL/6. Mainly, CD8 positive lymphocytes contacted with cultured epithelial cells of gallbladder. The biliary lesions developing spontaneously in these mice are similar to those found in human primary biliary cirrhosis.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Onji M,: "Spontaneous development of chronic nonsuppurative destructive cholangitis in senescent mice." Gastroenterol Jpn.26. 101- (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kanda K: "Spontaneous occurrence of autoimmune cholangitits in senescent mice." J Gastroenterol and Hepatol.7. (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] ONJI M: "Spontaneous development of Chronic non-suppurative destructive cholangitis in senescent mice." Gastoroenterologia Jpn. 26. 101 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] KANDA K: "Sponatenous occurrence of autoimmune cholangitis in senescent mice" J Gastroenterol and Hepatol. 7. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kanda K: "Spontaneous occurrence of autoimmune cholangitits in senescent mice." J Gastroenterol and Hepatol. (in press). 7. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 恩地 森一.: "実験的胆管消失症候群-老化マウス-" 肝胆膵. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Morikazu Onji et al: "Spontaneous Development of Chronic Nonーsuppurative Destructive Cholangitis in Senescent Mice" Gastroenterologia Japonica. 26. 101 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 恩地 森一,他: "老化マウスの胆管炎と原発性胆汁性肝硬変病変との類似性に関する病理組織学的検討" 消化器と免疫. 25. 190-192 (1991)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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