Study on the mechanisms of shedding of CEA into the serum by experimental system
| Project/Area Number |
03670369
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Sapporo Medical College |
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Principal Investigator |
HOSHI Hideki Sapporo Med. College, Dept. of Int. Med., Instructor, 医学部, 助手 (90219163)
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| Co-Investigator(Kenkyū-buntansha) |
YACHI Akira Sapporo Med. College, Dept. of Int. Med., Prof., 医学部, 教授 (50045324)
IMAI Kohzoh Sapporo Med. College, Dept. of Int. Med., Assoc. Prof., 医学部, 助教授 (60117603)
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1992: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1991: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | CEA / Mechanisms of shedding / Membrane antigen / Fight junction / Monoclonal antibody / tight junciton / インタ-フェロン / 動物モデル |
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| Research Abstract |
Expression of mRNAs of carcinoembryonic antigen (CEA) and nonspecific cross-reacting antigen (NCA) genes in colorectal carcinomas and adenomas was investigated by in situ hybridization (ISH) with specific biotinylated probes. Hybridization was clearly detected throughout the cytoplasm of 7 out of 15 adenomas and 13 out of 15 carcinomas with the CEA cDNA probe, and in 6 out of 15 adenomas and 10 out of 15 carcinomas with the NCA cDNA probe. The intensity of signal appeared to be stronger in carcinomas than that in adenomas, and the CEA and NCA mRNAs were expressed together in most of positive tissue specimens. On the other hand, noninvaded tissues adjacent to the carcinoma did not show any signal except for 4 cases faintly stained with the NCA probe. This finding was partly confirmed by Northern blot analysis which indicated that the specific bands for the CEA and NCA mRNAs were more intense in RNAs from carcinoma tissues than in those from adjacent noninvaded tissues. These data suggest that the ISH technique with biotinylated probes could be of use for analyzing expression and localization of CEA and related genes on tissue sections.
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Report
(3 results)
Research Products
(6 results)
