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Measurement of rat gastric mucus secretion using ex-vivo gastric chamber system

Research Project

Project/Area Number 03670377
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Gastroenterology
Research InstitutionKitasato University

Principal Investigator

ISHIHARA Kazuhiko  (Kitasato Univ., Sch. of Medicine, Associate Professor), 医学部, 助教授 (10104530)

Co-Investigator(Kenkyū-buntansha) ICHIKAWA Takafumi  in 1992, (Kitasato Univ., Sch. of Medicine, Research Associate), 医学部, 助手 (30245378)
KOMURO Yuichi  in 1991, (Kitasato Univ., Sch. of Medicine, Research Associate), 医学部, 助手 (20215411)
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1992: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1991: ¥600,000 (Direct Cost: ¥600,000)
KeywordsEx-vivo gastric chamber / Gastric mucus secretion / Gastric mucin determination / Hexosamine determination / Anti-mucin antibody / Monoclonal antibody / ELISA / Carbachol / 胃ムチン微量定量法 / ヘキソサミン微量定量法 / Exーvivoガストリックチャンパ- / 胃粘液 / ムチン / 胃分泌機構 / ラット胃粘膜
Research Abstract

For the assessment of gastric mucus secreted from rat gastric mucosa into gastric lumen, we examined an ex-vivo gastric chamber system which had been developed by Takeuchi et al. To apply this system for the measurement of mucin secreted into the effluent solution, we had to develop a highly sensitive method to determine gastric mucin content. Finally we adopted an HPLC method to determine the content of hexosamine which constitutes the major carbohydarate component of mucin molecule. By using this method coupled with the removal treatment of low molecular weight hexosamine-containing materials including non-mucinous glycoproteins, it could be achieved to measure the time course changes of mucin secreted into the effluent solution. This method was applied to estimate the effect of carbachol administration on the mucus secretion in the rat gastric mucosa, and obtained a result that this drug to be an accelerator of mucus secretion into the gastric lumen.
In the course of this study, we tried to obtain some monoclonal antibodies(MAbs) which are specific for the gastric mucin, and utilize them to the immunohistochemical method to determine the mucin content. We established several MAbs which are specific for the carbohydrate moieties of mucins present in the specific region of rat gastric mucosa. At present, we are trying to establish a method to assess the content of mucin originated from a specific site of gastric mucosa such as surface mucous cells, corpus gland mucous cells, antral gland mucous cells.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] KOMURO,Yuichi: "A separating method for quantifying mucus glycoprotein localized in the different layer of rat gastric mucosa" Gastroenterologia Japonica. 27. 466-472 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] KOMURO,Yuichi: "Effects of tetragastrin on mucus glycoprotein in rat gastric mucosal protection" Gastroenterologia Japonica. 27. 597-603 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] KOJIMA,Yoji: "Effects of the M_1 muscarinic receptor antagonist pirenzepine on gastric mucus glycoprotein in rats with or without ethanol-induced gastric damage" Scandinavian Journal of Gastroenterology. 27. 764-768 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] ISHIHARA,Kazuhiko: "Effect of rebamipide on mucus secretion by endogenous prosta-glandin-indepedent mechanism in rat gastric mucosa" Arzneimittel-Forschung/Drug Research. 42(II). 1462-1466 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] ISHIHARA,Kazuhiko: "Comparison of the mucus glycoproteins present in the different layers of rat gastric mucosa" Comparative Biochemistry and Physiology. 104B. 315-319 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] ISHIHARA,Kazuhiko: "Development of an HPLC method to estimate hexosamines and its application to determine mucin content in rat and human gastric mucosa" Comparative Biochemistry and Physiology. (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 石原 和彦(分担執筆,9頁分): "H_2受容体拮抗剤の諸作用とその問題点" 医学図書出版株式会社, 410 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 堀田 恭子・石原 和彦(共著): "胃粘液研究への招待" メジカルビュー社, 80 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] KOMURO,Y., Ishihara,K., Ota,H., Katsuyama,S., Saigenji,K. and Hotta,K.: "A separating method for quantifying mucus glycoprotein localized in the different layer of rat gastric mucosa" Gastroenterol Jpn. 27. 466-472 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] KOMURO,Y., Ishihara,K., Ohara,S., Saigenji,K. and Hotta,K.: "Effects of tetragastrin on mucus glycoprotein in rat gastric mucosal protection" Gastroenterol Jpn. 27. 597-603 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] KOJIMA,Y., Ishihara,K., Ohara,S., Saigenji,K. and Hotta,K.: "Effects of the M_1 muscarinic receptor antagonist pirenzepine on gastric mucus glycoprotein in rats with or without ethanol-induced gastric damage" Scand J Gastroenterol. 27. 764-768 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] ISHIHARA,K., Komuro,Y., Nishiyama,N., Yamasaki,K. and Hotta,K.: "Effect of rebamipide on mucus secretion by endogenous prostaglandin-independent mechanism in rat gastric mucosa" Arzneim-Forsch/Drug Res. 42(II). 1462-1466 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] ISHIHARA,K. and Hotta,K.: "Comparison of the mucus glycoproteins present in the different layers of rat gastric mucosa" Comp Biochem Physiol. 104B. 315-319 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] ISHIHARA,K., Kameyama,J. and Hotta,K.: "Development of an HPLC method to estimate hexosamines and its application to determine mucin content in rat and human gastric mucosa" Comp Biochem Physiol. part B. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] KOJIMA,Y., Ishihara,K., Komuro,Y., Saigenji,K. and Hotta,K.: "Effects of the muscarinic receptor agonist carbachol and/or antagonist pirenzepine on gastric mucus secretion in rats" Scand J Gastroenterol. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] KOMURO, Yuichi: "A separatiog method for quantifying mucus glycoprotein localized in the different layer of rat gastric mucosa" Gastroenterologia Japonica. 27. 466-472 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] KOMURO, Yuichi: "Effects of tetragastrin on mucus glycoprotein in rat gastric mucosal protection" Gastroenterologia Japonica. 27. 597-603 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] KOJIMA, Yoji: "Effects of the M_1 muscarinic receptor antagonist pirenzepine on gastric mucus glycoprotein in rats with or without ethanol-induced gastric damage" Scandinavian Journal of Gastroenterology. 27. 764-768 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] ISHIHARA, Kazuhiko: "Effect of rebamipide on mucus secretion by endogenous prosta glandin-indepedent mechanism in rat gastric mucosa" Arzneimittel-Forschung/Drug Research. 42(II). 1462-1466 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] ISHIHARA, Kazuhiko: "Comparison of the mucus glycoproteins present in the different layers of rat gastric mucosa" Comparative Biochemistry and Physiology. 104B. 315-319 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] ISHIHARA, Kazuhiko: "Development of an HPLC method to estimate hexosamines and its application to determine mucin content in rat and human gastric mucosa" Comparative Biochemistry and Physiology. 1. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 石原 和彦(分担執筆,9頁分): "H_2受容体拮抗剤の諸作用とその問題点" 医学図書出版株式会社, 405 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 堀田 恭子・石原 和彦(共著): "胃粘液研究への招待" メジカルビュー社, 80 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Y.Komuro,K.Ishihara,S.Ohara,K.Saigenji K.Hotta: "A new method of separation and guantifation of macus glycoprotein in rat gastric mucus gel layer and its application to mucus secretion induced by 16,16ーdimethyl PGE_2" Gastroen Teralogia Japonica. 26. 582-587 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Ichikawa,K.Ishihara,K.Saigenji,K.Hotta: "Effects of omeprazole on gastric muws glycoprotein biosynthesis" Gastroenteralogia Japonica. 26. 390 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Y.Komuro,K.Ishihara,K.Ishii,H.Ota,T.katsnyama,K.Saigenji,K.Hotta: "A separating method for guantifying mucus glycoprotein localized in the different layer of rat gastric mucosa" Gastroenterologia Japonica. 27. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Y.Komuro,K.Ishihara,S.Ohara,K.Saigenji,K.Hotta: "Effects of tetragastrin on mucus glycoprotein in rat gastric mucosal protection" Gastroenterologia Japonica. 27. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 石原 和彦(共著)(編集:岡部治彌・竹本忠良・西元寺克禮): "消化性潰瘍と防御因子増強剤このうちII防御因子1粘液の章(14ペ-ジ)を分担" 南江堂, 207 (1991)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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