| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1992: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1991: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Research Abstract |
Although the hepatic microcirculation is thought to be closely related to the liver function, there has been much controversy about the effect of ethanol on microcirculation. The present study was undertaken to quantitate changes in hepatic microcirculation after acute ethanol administration. To that effect, male rats were anesthetized with sodium pentobarbital(35 mg/kg bw) intraperitoneally. After laparotomy, a lobe of the liver was exposed on the slide glass at the window of metal plate for observation with an intravital inverted microscope assisted by SIT (silicon intensified target) camera. Erythrocytes were labeled with fluorescein isothiocyanate (FITC) according to the method of Zimmerhackl et al (1983), and injected from the catheter placed in the left carotid artery. 20% ethanol and 40% (3.0 g/kg bw and 6.0, respectively) were then injected through the stomach tube. The fluorescent microscopic image of the hepatic microcirculation was recorded on a videotape. The flow velocity
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of individual RBCs in sinusoids was measured with multipurpose computerized image analysis system for microcirculation (IM-Capiflow, Sweden) from playback of recorded images. Portal pressure, central vein pressure and mean arterial pressure were measured with the catheter cannulated in portal vein, central vein and the left carotid artery respectively. The flow velocity increased about 72% at 10-20 min after injection of 20"% ethanol and remained increased up to 60 min. But, the flow velocity after 40% ethanol decreased about 30 min after injection. Portal pressure increased about 18% and 24% after 20% ethanol and 40% respectively, but central vein pressure and mean arterial pressure had no significant change. In summary, acute oral administration of 20% ethanol increased portal pressure and the blood flow in hepatic microcirculation. On the other hand, that of 40% ethanol increased portal pressure but decreased the blood flow in hepatic microcirculation. These results suggested that microcirculatory disturbance was caused by changes in the liver induced by ethanol at high concentration. Less
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