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Studies on the Mechanisms of Regulation System on Albumin and AFP production in Human Hepatocellular Carcinoma Cell Lines

Research Project

Project/Area Number 03670382
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Gastroenterology
Research InstitutionThe Jikei University School of Medicine

Principal Investigator

HASUMURA Satoshi  The Jikei University School of Medicine, Internal Medicine(I), Medical Member, 医学部, 助手 (30189518)

Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1992: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1991: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsalbumin / AFP / human hepatocellular carcinoma / hyperthermia / thyroid hormone / steroid hormone / サイロドホルモン / スイッチング / ホルモン / サイトカイン
Research Abstract

It is well known that AFP level in hepatocellular carcinoma(HCC) serum frequently increases and after the birth, serum albumin appears rapidly and increases very fast. Synthesis of albumin and AFP in hepatocyte is deeply related to differentiation of parenchymal cell, liver regeneration and carcinogenesis.
These two genes of albumin and AFP are on the same chromosome and have same genetical origin. Some investigators report that each gene has enhancer gane and promoter genes and AFP gene has silencer legion on the upstream of promoter gene of AFP. These have important role for regulation of these proteins.
In this study, we establish human HCC cell lines which remain hepatocyte specific function and observe the effects of culture condition to synthesis of Albumin and AFP. We established 6 HCC cell lines and 3 of which were cultured in FCS free medium and produced albumin and/or AFP. We showed that these albumin and AFP were same as serum albumin and AFP immunologically and biochemically. Especially, AFP heterogeneity using lectin affinity was similar to serum AFP of HCC patient.
Furthermore,albumin secreted from JHH-4 cell was predominant at hyperthermic condition of 38-40 ゚C. On the other hand, thyroid hormone suppressed AFP production of JHH-7 cell and total protein, albumin and AFP synthesis increased addition of glucocorticoid to medium.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] 蓮村 哲,永森 静志,松浦 知和他: "無血清培養ヒト肝癌細胞株の産生する肝特異蛋白の解析" 日本消化器病学会雑誌. 88. 511- (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 新谷 稔,蓮村 哲,永森 静志他: "ヒト培養肝癌細胞株JHH-7の樹立とその多様な肝特異蛋白の産生" HUMAN CELL. Suppl.4(3). 57- (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 新谷 稔,蓮村 哲,永森 静志他: "ヒト肝癌由来細胞株JHH-7の樹立と温熱によるその蛋白産生性の変化" 日本癌学会総会記事(第50回). 155- (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 新谷 稔,蓮村 哲,永森 静志他: "ヒト由来肝癌細胞株JHH-7の肝特異蛋白産生に対する加温の影響" 組織培養研究. 11. 88- (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 新谷 稔,蓮村 哲,永森 静志他: "温熱処理下におけるヒト由来肝癌細胞株JHH-7の肝特異蛋白産生性の変化" HUMAN CELL. 5(3)Suppl. 30- (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 深谷 憲一,永森 静志,蓮村 哲他: "AFPアルブミン産生におよぼすステロイドホルモン,甲状腺ホルモンの影響" 肝臓. (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 蓮村 哲,永森 静志他: "ヒトがん細胞株とその特性" 中外医学社, 472 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] S.Nagamori,S.Hasumura,S.Nagase et al.: "Relation between Albumin-Positive Hepatocytes and Glutathione-S-Transferase-Positive Foci in Nagase Amalbuminemic Rats Treated with 3'-Methyl-4-Diaminoazo-benzen" Journal of Toxicologic Pathology. 5(1). 39-46 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 新谷 稔,蓮村 哲,永森 静志 他: "ヒト由来肝癌細胞株JHH-7の肝特異蛋白産生に対する加温の影響" 組織培養研究. 11. 88 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 新谷 稔,蓮村 哲,永森 静志 他: "温熱処理下におけるヒト由来肝癌細胞株JHH-7の肝特異蛋白産生性の変化" HUMAN CELL. 5(3)Suppl. 30 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 深谷 憲一,永森 静志,蓮村 哲 他: "AFP・アルブミン産生におよぼすステロイドホルモン,甲状腺ホルモンの影響" 肝臓. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 蓮村 哲,永森 静志他: "ヒトがん細胞株とその特性" 中外医学社, 472 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 蓮村 哲: "無血清培養ヒト肝癌細胞株の産生する肝特異蛋白の解析" 日本消化器病学会雑誌. 88. 511 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 新谷 稔: "ヒト培養肝癌細胞株JHHー7の樹立とその多様な肝特異蛋白の産生" HUMAN CELL. 4. 57 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 新谷 稔: "ヒト肝癌由来細胞株JHHー7の樹立と温熱による.その蛋白産生性の変化" 第50回日本癌学会総会抄録集. (1991)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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