Investigations of immunological disturbance in ulcerative colitis and animal models of inflammatory bowel disease.
| Project/Area Number |
03670386
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | KITASATO INSTITUTE |
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Principal Investigator |
TSUCHIMOTO Kanji Kitasato Institute Hospital, 病院, 内科部長 (70155399)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Noriaki Kitasato Institute Hospital, 病院, 内科医長 (80167155)
HIBI Toshifumi Keio Cancer Center, 診療部長 (50129623)
SUZUKI Tatsuo Kitasato Institute Research, 病院研究部, 部長 (40072409)
YAMADA Takaya Kitasato Institute Hospital, 病院バイオメディカル, 主任 (50191317)
HAMADA Yoshiki Kitasato Institute Hospital, 病院, 内科医長 (00137994)
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1992: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1991: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | ulcerative colitis / Crhon's disease / hapten / animal model of colitis / 実験腸炎 / 大腸特異抗原 |
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| Research Abstract |
It has been suggested that systemic and local immunological disorders play an important role in the pathogenesis of Crohn's disease and ulcerative colitis. Recently, hapten, 2,4,6-trinitrobenzene sulfonic acid(TNB) and sulfhydryl blocker, N-ethylmaleimide(NEM), have been found to induce chronic colitis. In this study, immuno-logical changes were analyzed in TNB and NEM-induced colitis. Following results were obtained. 1) TNB enema caused chronic ulceration in colon. A large number of neutrophils, macrophages and T cells were infiltrated in colonic ulcer. The number of neutrophils, macrophages, T cells, especially helper T cells were increased around ulcer, but the population of IgG containing cells were decreased. Several granulomas were found in serosal side of TNB colitis. 2) In rats immunized with TNB-BSA before TNB enema, the size of colonic ulcer was much greater than that in nonimmunized rats. However, there was no significant difference in the population of T cell subsets between immunized and nonimmunized rats. 3) NEM enema also induced ulcer similar to that with TNB. The patterns of infiltrating cells in and around ulcer were also similar to these in TNB colitis. But the number of IgA and IgG containing cells was markedly increased. The crypt abscess was found in NEM colitis. These results indicate that TNB colitis resembles Crohn's disease and NEM colitis resembles ulcerative colitis. These models for colitis might be useful for the establishment of treatment of inflammatory bowel disease.
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Report
(3 results)
Research Products
(3 results)
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[Publications] Hosoda,Y., Hibi,T., Ohohara,M., Hamada,Y., Tsuchimoto,K., Yamada,T., Suzuki,T., Iwao,Y., Hata,J., Tsuchiya,M.: "Movement of Immunocompetent Cells in Experimental Colitis.(lst report)" Digestive Organ and Immunology. 26. 68-72 (1992)
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