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Identification of histaminergic fiber in human lung

Research Project

Project/Area Number 03670389
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Respiratory organ internal medicine
Research InstitutionTOHOKU UNIVERSITY

Principal Investigator

YAMAUCHI Kohei  Tohoku Univ. School of Medicine, 医学部, 助手 (20200579)

Co-Investigator(Kenkyū-buntansha) OHTANI Haruo  Tohoku Univ. School of Medicine, 医学部, 助手
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1992: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1991: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsL-histidine decarboxylase (HDC) / cDNA / Basophils / alternative splicing / mRNA / KU-812F / L-histidine decarboxylase(HDC) / KU-812-F / ヒスタミン / LーHisTidine Decarboxylase(HDC) / 抗体
Research Abstract

The primary structure of HDC from KU-812F was determined by the analysis of the amino acid sequence deduced from the nucleotide sequence of the human HDC cDNA clone(p-TN2)encoding functional HDC. Human HDC has 662 amino acids(Mr 74,178)and shows 85% homology in amino acid sequence to the rat and mouse HDC sequences. 74kDa premature form of human HDC was demonstrated by in vitro translation from HDC mRNA,suggesting that human HDC is processed post-translationally. KU-817F expresses 2.4-kb and 3.4-kb HDC mRNA's which are alternatively spliced from the HDC gene. Among these mRNA's,only 2.4-kb mRNA encodes functional HDC.In human tissues including the lung and brain,the expression of 2.4-kb HDC mRNA was evaluated. While HDC gene expression was low in the lungs of normal individual,or not detected in the lungs from the patients with IPF,the HDC mRNA level was markedly increased in the lungs from the patients with bronchial asthma,suggesting that hyperproduction of histamine may be involved in the pathogenesis of bronchial asthma.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] K.Yamauchi,R.Mamune-Sato,Y.Tanno et al.: "Molecular biological aspect of human L-histidine decarboxylase (1993 "Histamine in normal and cancer cell proliferation. Its importance in basic and clinical research" M Garcia-Caballero,L J Brandes and S Hosoda eds, Pergamon Press Oxford"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] R.Mamune-Sato,Y.Yamauchi,Y.Tanno et al.: "Functional analysis of alternatively spliced transcripts of the human histidine decarboxylase gene and its expression in human tissues and basophilic leukemia cells." Eur J Biochem. 209. 533-539 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] K.Sekizawa,H.Nakazawa,T.Ohrui,K.Yamauchi et al.: "Histamine N-methyl-transferase modulates histamine- and antigen- induced bronchoconstriction in guinea pigs in vivo." Am Rev Respir Dis. 147. 92-96 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Y.Ohkawara,Y.Yamauchi,Y.Tanno et al.: "Human lung mast cells and pulmonary macrophages produce tumor necrosis factor-a in sensitized lung tissue after IgE receptor triggering." Am J Respir Cell Mol Biol. 7. 385-392 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Ohrui,Y.Yamauchi,K.Sekizawa et al.: "Histamine N-methyltransferase controls the contractile response of guinea pig trachea to histamine" J Pharmacol Ext Ther. 261. 1268-1272 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Ohrui,K.Sekizawa,Y.Yamauchi et al.: "Chemical oxidant potentiates electrically and acetylcholine- induced contraction in rat trachea:Possible involvement of cholinesterase inhibition" J Pharmacol Exp Ther. 259. 371-376 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] R.Mamune-Sato,K.Yamauchi,Y.Tanno,Y ohkawara et al: "Functional analysis of alternatively Spliced Transcripts of the human histidine decarboxylase gene and its expression in human tissues andbasophylic leukemiaCells" Eur.J.Biochem.209. 533-539 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Y.Ohkawara,K.Yamauchi,Y.Tanno,G.Tamura,H.Ohtani et al: "Human lung mast cells and pulmonary macrophages procluce tumor necrosis factor α in sensitized lung tissue after IgEreceptor triggering" Am.J.Respir.Cell.Mol.Biol. 7. 385-392 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] K.Sekizawa,H.Nakazawa,T.Ohrui,K.Yamauchi et al: "Histamine N-metlyltransterase modulate histamine-and antigen-induced bronchoconstriction in guinea pid in vivo" Am.J.Respir.Dis. 147. 92-96 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] K.yamauchi,R.Mamune-Sato,Y.Tanno et al: "Histamine in normal and cancer cell proliferation Its importance in basic and clinical rosearch-Molecular biological aspect of human L-histidine decarboxylase" Pergamon Press(Oxford), (1993)

    • Related Report
      1992 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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