| Project/Area Number |
03670396
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | SHINSHU UNIVERSITY SCHOOL OF MEDICINE |
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Principal Investigator |
YOSHIMURA Kazuhiko Shinshu Univ., Assistant Prof., 医学部附属心脈管病研究施設, 講師 (70174985)
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| Co-Investigator(Kenkyū-buntansha) |
KUBO Keishi Shinshu Univ., Assistant Prof., 医学部, 講師 (80143965)
KOBAYASHI Toshio Shinshu Univ., Assistant Prof., 医学部附属病院, 講師 (80020775)
HANDA Kenjiro Shinshu Univ., Associate Prof., 医学部, 助教授 (70020724)
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1992: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1991: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | chemotactic factor / leukotriene B4 / polymorphonuclear leukocyte / pulmonary vascular permeability / filtration coefficient / isolated perfused lungs / wet to dry lung weight ratio / pulmonary edema / ロイコトリエンB_4 / 摘出潅流肺 / 肺温乾重量比 |
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| Research Abstract |
The effects of exogenous LTB4 on the pulmonary microvascular perme-ability and the roles of polymorphonuclear leukocytes (PMNs), cyclooxy-genase products of arachidonic acid and neutrophil elastase in the microvascular response to LTB4 in the isolated non-blood perfused rabbit lungs were studied. We observed that LTB4 increased filtration coefficient (Kf) and wet to dry lung weight ratio (W/D ratio) in the presence of PMNs in the perfusate. Neither LTB4 alone nor PMNs alone produced changes in Kf and W/D ratio. Indomethacin failed to inhibit the LTB4-induced increases in Kf and W/D ratio. Neutrophil elastase inhibitor effectively inhibited the LTB4-induced lung injury. These results suggest that LTB4 produces lung injury which is dependent on neutrophil elastase derived from PMNs but not on the cyclooxygenase pathway of arachidonic acid metabolism.
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