| Project/Area Number |
03670399
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
NAGAI Sonoko Chest Disease Clinical Immunology Research Institute Associate Professor, 胸部疾患研究所, 助教授 (30217955)
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1992: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1991: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Alveolar macrophage / IL-1 / IL-1 inhibitory factor / IL-1 receptor antagonist / PCR / HPLC / Sarcoidosis / Idiopathic Pulmonary Fibrosis / 1L-1 / 1L-1抑制物質 / 1L-1レセプターアンタゴニスト / 肺胞マクロファ-ジ / インタ-ロイキン1 / インタ-ロイキン1抑制物質 / レセプタ-・アンタゴニスト / 慢性炎症 |
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| Research Abstract |
We tried to examine whether an unbalance between IL-1 and IL-1 inhibitor production from alveolar macrophages in patients with interstitial lung diseases or healthy smokers, compared with healthy nonsmokers,because such unbalance may contribute to a development of chronic low grade inflammation in lungs.
The results were:
1. Increase in IL-1 activities and significant decrease in IL-1 inhibitor were detected in the culture supernatants of alveolar macrophages from patients or smokers.
2. IL-1 inhibitor was identified as IL-1 receptor antagonist by using receptor binding assay.
3. IL-1 and IL-1 receptor antagonist gene expression were examined by using reverse-PCR method, and standardized by expressing the ratio to house keeping gene(G6-PD). The results showed a decrease in the expression of IL-1 receptor antagonist in patients or smokers, compared with healthy nonsmokers. Decreased expression of IL-1 receptor antagonist may have a role of developping chronic inflammatory process in lungs in patient with interstitial lung diseases and smokers.
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