| Project/Area Number |
03670400
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Osaka University |
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Principal Investigator |
KAWASE Ichiro (1992) The Department of internal Medicine III, Osaka University Medical School, Assistant Professor, 医学部第3内科, 講師 (10161324)
桝野 富彌 (1991) 大阪大学, 医学部・第3内科, 講師 (20135677)
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| Co-Investigator(Kenkyū-buntansha) |
SAITO Shinichi Osaka university Hospital, Post Doctoral Fellow, 医学部附属病院, 医員
川瀬 一郎 大阪大学, 医学部・第3内科, 助手 (10161324)
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1992: ¥300,000 (Direct Cost: ¥300,000)
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| Keywords | Small cell lung cancer / Neural cell adhesion molecule / N-CAM / Monoclonal antibody |
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| Research Abstract |
Murine lgG1 monoclonal antibody (mAb) designated ITK-2 was generated against a small cell lung cancer (SCLC) cell line. ITK-2 reacts with neuroendocrine tissues and tumors including SCLC, and recognize a 145kDa glycoprotein of SCLC cell membrane extracts, called neural cell adhesion molecule (N-CAM). This result was correlated with Northern blotting where a single 6.2 kb mRNA was detected in SCLC cell lines. To determine cDNA sequence encoding the N-CAM isoform, several cDNA clones were isolated from OS2-R, a SCLC cell line established in our laboratory. Based on the analysis of the full-length cDNA obtained from two clones, the sequence of this 145 kDa isoform was shown to be essentially identical to that of the 140 kDa N- CAM isoform of neuroblastoma except two differences which include the lack of 30-bp insert termed the VASE exon and a single base pair change at position 1620 without changing encoding amino acid.
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