| Project/Area Number |
03670410
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Tokyo Metropilitan Institute of Gerontology |
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Principal Investigator |
KIDA Kozui Tokyo Metropolitan Institute of Gerontology, Department of Immmunopathology, Researcher, 免疫病理, 研究員 (90142645)
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| Co-Investigator(Kenkyū-buntansha) |
HIRATSUKA Tomoko Tokyo Metropolitan Institute of Gerontology, Department of Immmunopathology, 免疫病理, 研究員 (30238373)
JINNO Satoru Tokyo Metropolitan Institute of Gerontology, Department of Clinical Biochemistry, 酸素生化学, 研究員
MIZUUCHI Tomoko Tokyo Metropolitan Institute of Gerontology, Department of Clinical Pathology, R, 臨床病理, 研究員
柱 秀樹 東京都老人総合研究所, 免疫病理部門, 研究員
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1992: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1991: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | Hyperoxia / lung injury / tissue repair / bleomycin injecti-ons / DNA量 / 高濃度酵素 / DNA含有量 / DNA修復,傷害 / 肺線維化 / DNA polymerse |
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| Research Abstract |
1. Changes in the DNA content and the activity of DNA polymerase and its alpha and beta forms were studied on the lungs, which were exposured in hyperoxia, of one day prenatal to 42 day postnatal rats. It is concluded that : (1) cellular proliferation in the lung is most active during the first two weeks after birth as supported by increase in both DNA polymerase alpha activity and DNA content, and (2) the capacity for DNA repair, DNA polymerase beta activity throughout the pre and postnatal period remains constant.
2. Changes in the DNA synthesis and cellular constituents of mouse lung following repeated bleomycin injections were studied. (1) repeated injections of BLM cause DNA injury in lung cells ; (2) there is a subsequent increase in the DNA repair function as supported by the finding of an increase in DNA polymerase-beta activity ; and (3) these lead further to cell prolifration as supported by the increase in both DNA polymerase-alpha activity and DNA content.
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