| Project/Area Number |
03670415
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurology
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| Research Institution | Fukui Medical School |
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Principal Investigator |
MUTOH Tatsuro Fukui Medical School Assit.Prof, 医学部, 助手 (60190857)
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| Co-Investigator(Kenkyū-buntansha) |
HAMAGUCHI Michinari Nagoya Univ.School of Medicine.Prof., 医学部, 教授 (90135351)
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| Project Period (FY) |
1991 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1992: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1991: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Differentiation / Proliferation / EGF / NGF / Trk / Tyrosine kinase / B subunit of cholera toxin / Gangliosides / 分化 / 増殖 / チロシンキナーゼ / コレラ毒素Bサブユニット / ガングリオシド / p140^<trk> / phoshorylation / tyrosine Kinese / Wastern blot / PC12 Cell / 細胞分化 / 細胞増殖 / キナ-ゼ / リン酸化 / サイトカラシンB |
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| Research Abstract |
[Objective]
To elucidate the role of endogenous gangliosides (Gg) on the process of cell differentiation and proliferation, we analyzed the effect of Gg on the activity of intracellular signal transduction pathways for cell differentiation and proliferation.
[Method]
As for cell proliferation, we examined the activity of intracellular signal transduction pathway of epidermal growth factor (EGF) in skin fibroblasts from patients with GM1 gangliosidosis and normal controls. We previously reported that GM1 ganglioside is accumulated in fibroblasts from GM1 gangliosidosis when compared with that of normal controls. As for cell differentiation, we examined the effect of B subunit cholera toxin (CTB)-pretreatment, which specifically binds to GM1 and causes the re-distribution of GM1 on the plane of the plasma membrances, on NGF-signaling pathway in PC12 cells.
[Results and Discussion]
GM1 was found to be the negative regulator of EGF receptor function by reducing EGF-binding and by inhibiting EGF receptor associated tyrosine kinase (TK) activity. On the other hand, CTB augmented the intracellular NGF-signaling pathway. Moreover, in Trk A-immune complex kinase assay, GM1 was found to be a potent activator of Trk A-associated TK activity that is senseitve to NGF.Thus, GM1 seems to be an endogenous stimulator for cell differentiation.
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