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In vitro model of blood-brain barrier in the autoimmune central nervous system disorders.

Research Project

Project/Area Number 03670416
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Neurology
Research InstitutionShinshu University

Principal Investigator

KOH Shosei  Shinshu University Hospital Department of Medicine (Neurology) Assistant Professor, 医学部・付属病院, 講師 (80143981)

Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1992: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1991: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsBlood-brain barrier / experimental autoimmune encephalomyelitis / multiple sclerosis / vascular endothelial cell / oligodendroglia / 血液脳関門 / 実験的自己免疫性脳脊髄点
Research Abstract

Experimental autoimmune encephalomyelitis (EAE) is an autoimmune diseaase of central nervous system (CNS) myelin which can be induced in various species by a single injection of CNS tissue or isolated myelin basic protein (MBP), in combination with appropriate immunological adjuvants. EAE provides a model neuroautoimmune animal disease with clinical and immunohistopathologic similarities to human demyelinating disorders, e.g., multiple sclerosis (MS). Considerable evidence exists that activated T lymphocytes, reactive with myelin in general and MBP in particular, represent an important immune effector mechanism responsible for the inflammatory events underlying EAE and MS.Two closely related and as yet unresolved issues concerning EAE and MS are : 1) the anatomic site at which the host immune effector initially affects brain and spinal cord target tissue and 2) the specific events following this initial effector-target interaction that result in the development of neurologic disease. To resolve this issues we have established in vitro model of blood-brain barrier (BBB) by culturing brain vascular endothelial cells and oligodendroglia cells. We have measured BBB permeability by nsing this in vitro model and studied effects of inflammatory cytokines on BBB permeability. We have found that some of inflammatory cytokines are increasing factrt of BBB permeability. Some of cytokines induced procoagulant and/or fibrinolytic enzymes and injured oligodendroglia.

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] K.Shimada: "Antilymphocyte antibodies and circulating immune complexes in the sera of patients with myelopathy associated with human T lymphotropic virus type-I." J.Neuroimmunol.42. 161-166 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] C.-S.Koh: "Nurovascular permeability and fibrln deposition in the central neuraxis of Lewis rats with cell-transferred experrimental allrgic encephalomyelitis in relationship to clinical and histopathologic features of the disease." J.Neuroimmunol.47. 141-145 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] C.-S.Koh: "Immunoadsorption plasmapheresis and plasma exchange in Miller Fisher syndrome." Therapeutic Plasmapheresis. 12. 557-560 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Shimada: "Suppression of experimeutal allergic encephalomyelitis in Lewis rats by administration of gangliosides." Cell.Immunol.154. 231-239 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] C.-S.Koh: "Immunoadsorption Plasmapheresis in polymyositis/dermatomyositis:beneficial effects in four cases." Therapeutic Plasmapheresis. 13(印刷中). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Sakai: "A neural protein associated with paraneoplastic ueurologic syndrome and small cell lung carcinoma derived the hippocampus." Biochem.Biophys.Res.Commun.(印刷中). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Shimada, C.-S.Koh, N.Yanagisawa, N.Tsukada, M.Osame: "Antilymphocyte antibodies and circu-lating immune complexes in the sera of patients with myelopathy associated with human Tlymphotropic virus type-I." J.Neuroimmunol.42. 161-166 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Shinoda, C.-S.Koh, H.Arakura, E.Yamagami, M.Katakura, N.Yanagisawa: "Immunoadsorption plasma pheresis and plasma exchange in Guillain-Barre syndrome." Therapeutic plasmapheresis. 12. 531-534 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] C.-S.Koh, T.Shinoda, K.Shimada, A.Inoue, N.Yanagisawa: "Immunoadsorption plasma pheresis and plasma exchange in Miller Fisher syndrome." Therapeutic Plasmapheresis. 12. 557-560 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] C.-S.Koh, J.Gausas and P.Y.Paterson: "Neuro-vascular permeability and fibrin deposition in the central neuraxis of Lewis rats with cell-transferred experimental allergic encephalo-myelitis in relationship to clinical and his-topathological features of the disease." J.Neuroimmunol.47. 141-145 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Shimada, C.-S.Koh, K.Uemura and N.Yanagisawa: "Suppression of experimental allergic encephalomyelitis in Lewis rats by administration of gangliosides." Cell.Immunol.154. 231-239 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] C.-S.Koh, K.Shimada, A.Inoue, Y.Shimizu, N.Yanagisawa, T.Shinoda, H.Arakura, H.Oguchi: "Immunoadsorption plasmapheresis in polymyo-sitis/dermatomyositis : beneficial effects in four cases." Therapeutic Plasmapheresis. (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Sakai M.Gofuku, Y.Kitagawa, T.Ogasawara, G.Hirose, M.Yamazaki, C.-S.Koh, N.Yanagisawa: "A neural protein associated with paraneoplastic neurologic syndrome and small cell Olung carcinoma derived the hippocampus." Biochem.Biophys.Res.Commun.(in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Shimada: "Anti-lymphocyte antibodies and circulating immune complexes in the sera of patients with myelopathy associated with human T lymphotropic virus type-I." J.Neuroimmunol.42. 161-166 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] C.-S.Koh: "Neurovascular permeability and fibrin deposition in the central neuraxis of Lewis rats with cell-transfferred experimental allergic encephalomyelitis in relationship to clinical and histopathologic features of the disease" J.Neuroimmunol.47. 141-145 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] K.Shimada: "Suppression of experimental allergic encephalomyelitis in Lewis rats by administration of gangliosides." Cell.Immunol.154. 231-239 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] C.-S.Koh: "Immunoadsorption plasmapheresis and plasma exchange in Miller Fisher syndrome." Therapeutic Plasmapheresis. 12. 557-560 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] C.-S.Koh: "Immunoadsorption plasmapheresis in polymyositis/dermatomyositis:beneficial effects in four cases." Therapeutic Plasmapheresis. 13 (印刷中). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] K.Sakai: "A neural protein associated with paraneoplastic neurologic syndrome and small cell lung carcinoma derived the hippocampus." Biochem.Biophys.Res.Commun.(印刷中). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] C.-S.Koh: "Concordance and localization of maximal vascular permeability change and fibrin deposition in the central neuraxis ofLewis rats with cell-transferred experimental allergic encephalomyelitis." J.Neuroimmunol.38. 85-94 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] K.Nakazawa: "An autopsy case of Crow-Fukase syndrome (POEMS) with a high level of IL-6 in the ascites." Acta Pathol.Jpn.42. 651-656 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] C.-S.Koh: "Two cases of Miller Fisher syndrome which were successfully treated with immunoadsorption plasmapheresis." Therapeutic Plasmapheresis. 10. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] K.Shimada: "Antilymphocyte antibodies and circulating immune complexes in the sera of patients with myelopathy associated with human T lymphotropic virus type-1." J.Neuroimmunol.42. 161-166 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] C.-S.Koh: "Immunoadsorption plasma pheresis and plasma exchage in Miller Fisher syndrome." Therapeutic Plasmapheresis. 11. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] C.-S.Koh: "Neurovascular permeability and fibrin deposition in the central neuraxis of Lewis rats cell-transferred experimental allergic encephalomyelitis in relationship to clinical and histopathological features of the disease." J.Neuroimmunol. 44. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kok,C.ーS.et al.: "Effects of cytokines in permeability of in vitro model of bloodーbrain barrier." Journal of Neuroimmunology.

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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