| Project/Area Number |
03670446
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
HAYASHI Hideharu Hamamatsu University School of Medicine Photon Medical Research Center Associate Professor, 光量子医学研究センター, 助教授 (50135258)
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| Co-Investigator(Kenkyū-buntansha) |
TERADA Hajime Hamamatsu University School of Medicine Department of Medicine Assistant, 医学部, 助手 (50252177)
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| Project Period (FY) |
1991 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1993: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1992: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1991: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | [Na^+]i / [Ca^<2+>]i / SBFI / fluo-3 / strophanthidin / metabolic inhibition / Na^+ / H^+ exchange / Ca^<2+> exchange / 蛍光色素 / Na^+-H^+交換 / Na^+-Ca^<2+>交換 / 細胞内Na^十濃度([Na^十]i) / 細胞内Ca^<2十>濃度([Ca^<2十>]i) / 蛍光色素法 / 細胞障害 / 虚血・再潅流 / 細胞内pH(pHi) / カルシウム過負荷 / Furaー2 / BCECF |
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| Research Abstract |
We monitored [Na^+]i and [Ca^<2+>]i simultaneously using SBFI/AM and fluo-3/AM in guinea pig ventricular myocytes. After the addition of 500 muM strophanthidin, [Na^+]i increased from 7.9(〕SY.+-.〔)0.4 mM (mean(〕SY.+-.〔)SE) to 19.6(〕SY.+-.〔)1.8 mM at 40 min. [Ca^<2+>]i was low during the first 20 min, and then began to increase to 361(〕SY.+-.〔)69 % of the control at 40 min. The addition of hexamethylene amiloride (HMA : 1muM) prevented the increases in both [Na^+]i and [Ca^<2+>]i. Ryanodine (1muM) suppressed the increase in [Ca^<2+>]i. These findings indicated that (1) the pathway of Na^+ entry was mainly through Na^+/H^+ exchange, and that the elevated [Na^+]i induced Ca^<2+> entry mediated by the Na+/Ca^<2+> exchange, (2) the entered Ca^<2+> triggered the Ca^<2+> release from the sarcoplasmic reticulum. For metabolic inhibition (MI), the perfusate contained 3.3 mM amytal and 5 muM CCCP.During the first 20 min of MI, [Na^+]i increased from 6.2(〕SY.+-.〔)0.5 mM to 18.6(〕SY.+-.〔)1.6 mM (p<0.01, m=31), whereas [Ca^<2+>]i remained at the low level. In the following 30 min, 29 of 31 (94 %) myocytes developed contracture, and [Ca^<2+>]i began to increase after cells had contracted until it reached to 167(〕SY.+-.〔)14 % of the control (p<0.01). The levels of [Ca^<2+>]i when cells contracted or hypercontracted during MI were much lower than those during 500 muM strophanthidin perfusion, whereas [Na^+]i increased further. It was shown that there was a dissociation in the relationship between [Na^+]i and [Ca^<2+>]i during MI.The increase in [Na^+]i during MI was suppressed by 1 muM HMA or 10 mM glucose. The addition of 10 mM glucose after 20 min of MI led to a dramatic increase in [Ca^<2+>]i to 442(〕SY.+-.〔)72 % of the control (50 min. n=31, p<0.01). The increase in [Ca^<2+>]i and cell contracture after energy repletion were suppres
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