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Basic Aspects of Susceptibility to Seizures in Childhood Epilepsy

Research Project

Project/Area Number 03670504
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Pediatrics
Research InstitutionOsaka City University Medical School

Principal Investigator

MURATA Ryosuke  Osaka City University Medical School, Associate Professor of Pediatrics, 医学部, 助教授 (20128755)

Co-Investigator(Kenkyū-buntansha) MATSUOKA Osamu  Osaka City University Medical School, Lecturer in Pediatrics, 医学部, 講師 (20117972)
Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1992: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1991: ¥800,000 (Direct Cost: ¥800,000)
KeywordsAmygdala kindling / Suckling rats / Entorhinal response / Phosphatidylinositol / Ibotenate / Childhood epilepsy / NMDA受容体 / 痙攣準備性 / 幼若ラット / 興奮性シナプス / 長期増強作用 / 短期増強作用 / イノシト-ルリン脂質
Research Abstract

The experimental model of childhood epilepsy involves kindling of rats, in which repeated electrical stimulation of the brain (usually the amygdala) leads to focal afterdischarges (ADs) and then generalized seizures. We studied the relationship between the duration of ADs and changes in entorhinal responses during amygdala kindling in suckling rats (9-29 days old) in a physiological study, and then investigated the mechanism of kindling by administering ibotenate in a biochemical study of this phenomenon.
In the first study, the entorhinal field potential was recorded with a tungsten wire electrode during amygdala stimulation of anesthetized rats. As rats developed, the amplitude and the latency of the first peak of the negative component increased and decreased, respectively. The half-width of this component decreased. As ADs after a kindling stimulation appeared, this half-width increased. Thus, changes in the entorhinal response were seen as an increase in its half-width as kindling developed.
The second study was done by stimulation of receptors for the excitatory amino acid ibotenate by its administration, which caused phosphatidylinositol (PI) hydrolysis. The effect decreased with the age of the suckling rats. Kindling caused increased hydrolysis. Thus, kindling may reactivate receptors during development, allowing renewed PI hydrolysis. Perhaps increased efficacy of synaptic transmission accounts for the changes observed during kindling and long-lasting susceptibility to seizures.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report

Research Products

(10 results)

All Other

All Publications (10 results)

  • [Publications] 平山 謙.他: "ラット辺緑茶におけるイノシトールリン脂質代謝と扁桃核電気刺激による嗅内野誘発電位の生後発達" 神経組織の成長・再生・移植. 3. 108-109 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 宮城 伸浩.他: "幼若ラットキンドリング中の誘発反応の変化と後発射発達との関係" 大阪市医学会雑誌. 41. 155-169 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hirayama K,et al: "Effect of N-methyl-D-aspartate antagomit on entcrhinal responses by amygdala kindling stimulations" Jpn J Psychiatr Neurol. 46. 521-522 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hirayama K, Miyagi N, Nakajima S, Murata R, Matsuura S.: "Developmental changes of phosphatidylinositol metabolism and entorhinal field potentials by amygdala stimulation in suckling rats." Japanese Society for Neural Growth, Regeneration and Transplantation. 3. 108-109 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Miyagi N, Murata R, Matsuura S.: "Developmental changes in entorhinal responses and afterdischarges during amygdala kindling in growing rats." The Journal of The Osaka City Medical Center. 41. 155-169 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hirayama K, Miyagi N, Nakajima S, Kawawaki H, Murata R, Matsuura S.: "Effect of N-methyl-D-aspartate antagonist on entorhinal responses by amygdala kindling stimulations." Jpn J Psychiatr Neurol. 46. 521-522 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 宮城 伸浩.他: "幼若ラットキンドリング中の誘発反応の変化と後発射発達との関係" 大阪市医学会雑誌. 41. 155-169 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hirayama K,et al: "Effect of N-methyl-D-dspartate dntagcnist on entorhinal responses by dmygdald Kindling stimilations" Jpn J Psychidtr Neurol. 46. 521-522 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 平山 謙: "ラット辺線系におけるイノシト-ルリン脂質代謝と扁桃核電気刺激による嗅内野誘発電位の生復発達" 神経組識の成長・再生・移植. 3. 108-109 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hirayama K: "Effect of N‐methuyl‐D‐aspartate antagonist on entorhinal response by amygdala kindling stimulations" The Japanese Journal of Psychiatry and Neurology. (1992)

    • Related Report
      1991 Annual Research Report

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Published: 1991-03-31   Modified: 2016-04-21  

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